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Record W4311681162 · doi:10.22215/etd/2022-15264

Brain Derived Neurotrophic Factor (BDNF) as a Mediator of Microglia-Induced Neuroinflammation

2022· dissertation· en· W4311681162 on OpenAlexaff
Tryston Charlton

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldNeuroscience
TopicNeuroinflammation and Neurodegeneration Mechanisms
Canadian institutionsCarleton University
Fundersnot available
KeywordsMicrogliaNeuroinflammationNeuroprotectionNeurotrophic factorsBrain-derived neurotrophic factorInflammationNeuroscienceMediatorNeurotrophinTumor necrosis factor alphaCrosstalkMedicineImmunologyBiologyCell biologyInternal medicineReceptor

Abstract

fetched live from OpenAlex

Microglia are the primary immunocompetent cells that protect the brain from environmental stressors; however, their activation can also have deleterious effects on brain functioning.Indeed, environmental toxins and microbial agents can induce microglial driven inflammatory processes that increase levels of pro-inflammatory cytokines and induce a cytotoxic environment.Recent therapeutic strategies have sought to determine how to modulate microglia, so as to favour their neuroprotective effects, while minimizing toxic outcomes.BDNF is one of the most commonly expressed neurotrophins in the brain and is important for the regulation of plasticity, synapse formation, and general neuron health.Yet, little is known about how exogenous BDNF directly effects microglial activity.We hypothesized that BDNF would have a modulatory effect on inflammation in isolated microglia cultures in the context of a bacterial endotoxin.To this end, we indeed found that a BDNF treatment following LPS-induced inflammation attenuated the release of both IL-6 and TNF-α in primary microglia.In neurons, LPS-activated microglial media was able produce a minor inflammatory response, while secondary treatment of BDNF reduced this effect.Interestingly, LPS activated microglial media alone was found to increase production of anti-inflammatory IL-4 in neurons.We speculate that BDNF plays a role in regulating microglia activation and localized microglia-neuron crosstalk may be crucial in preventing damaging effects of inflammatory mechanisms.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.033
GPT teacher head0.293
Teacher spread0.259 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes1
Has abstractyes

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