Highly efficient platelet generation in lung vasculature reproduced by microfluidics
Bibliographic record
Abstract
Abstract Platelets, small hemostatic blood cells, are derived from megakaryocytes (MKs). It is accepted that both bone marrow (BM) and lung are principal sites of thrombogenesis although underlying mechanisms remain unclear. Outside the body, however, our ability to generate platelets, and retain their functionality, is poor at present. Here we show that perfusion of MKs ex vivo through the mouse lung vasculature generates substantial platelet numbers, up to 3,000 per MK. Despite their large size, MKs were able repeatedly to passage through the lung vasculature, leading to enucleation and subsequent platelet generation intravascularly. Using the ex vivo lung and a novel in vitro microfluidic chamber we determined how oxygenation, ventilation and endothelial cell health support platelet generation. Our data also show a critical role for the actin regulator TPM4 in the final steps of platelet formation in lung vasculature. The findings could inform new approaches to large scale generation of platelets.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".