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Record W4312197266 · doi:10.1101/2022.12.22.521553

16s rRNA gene sequence analysis of the microbial community on microplastic samples from the North Atlantic and Great Pacific Garbage Patches

2022· preprint· en· W4312197266 on OpenAlexfundno aff
Dkawlma Tora, Ute Hentschel, S. H. Lips, Mechthild Schmitt‐Jansen, Erik Borchert

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2022
Typepreprint
Languageen
FieldEnvironmental Science
TopicMicroplastics and Plastic Pollution
Canadian institutionsnot available
FundersWest African Science Service Centre on Climate Change and Adapted Land UseMemorial University of NewfoundlandBundesministerium für Bildung und ForschungUniversidad del Atlántico
KeywordsOcean gyreExtreme environmentBiologyPlastic pollutionEcologyMicrobial population biologyPhylumProteobacteriaOceanographyAmplicon sequencing16S ribosomal RNAMicroplasticsSubtropicsBacteriaGeologyPaleontology

Abstract

fetched live from OpenAlex

ABSTRACT The exponential increase in plastic production has led to their accumulation in the environment, particularly in oceans, polluting these environments from the shore to the open ocean and even sea ice in the pole regions. We compared microbial communities on plastic particles, known as “Plastisphere”, collected from the Atlantic and Pacific oceans gyres in the Summer of 2019 and subsequently looked for potential plastic degraders. We applied a 16S rRNA amplicon sequencing approach to decipher differences and similarities in colonization behaviour between these two gyres. Two polymer types include plastics: polyethylene (PE) and polypropylene (PP). We found that microbes differed significantly between the two oceans and identified thirty-two differentially abundant taxa at the class level. Proteobacteria, Cyanobacteria and Bacteroidota were the most prominent relative abundant phyla in the two oceans. Finally, according to the current literature, we found 40 genera documented as potential plastic degraders. This study highlights the importance of the biogeographical location with respect to microbial colonization patterns of marine plastic debris, differing even in the open oceans. Furthermore, the wide distribution of potential plastic-degrading bacteria was shown.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.012
Threshold uncertainty score0.024

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.020
GPT teacher head0.194
Teacher spread0.174 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2022
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)Same topicMicroplastics and Plastic PollutionFrench-language works237,207