Molecular characters of Boleodorus thylactus (Nematoda) associated with avocado (Persea americana) using ITS rDNA from regions of South Africa
Why this work is in the frame
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Bibliographic record
Abstract
Plant-parasitic nematodes are the main challenge for fruit production in Limpopo Province, South Africa. Besides, the family Tylenchidae are ectoparasite, affecting the yield in a high population. Boleodorus species are not being reported from avocado in South Africa; therefore, its identification is vital to avocado farmers. Hence, molecular study for its accurate identification is needed. This molecular study was conducted in 2022 at the University of Limpopo to identify the nematodes from South African soil associated with crops and trees. First, the recovered nematode was extracted using the tray method, and then its DNA was extracted using the chelex method. Afterward, ITS rDNA was amplified using specific primers to identify the nematode. The Nblast analysis based on the ITS rDNA showed 100% similarity with a population of Boleodorus thylactus (MZ099822, MZ099823) from Canada. In addition, phylogenetic analysis using the maximum likelihood method as implemented in mega x software was done. The phylogenetic analysis put the South African populations of B. thylactus together with other B. thylactus with a 100-bootstrap value. In conclusion, this species' diversity needs to be investigated to determine the impact of this nematode on the avocado yield.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it