ERK1/2 and NFκB are key signaling pathways in the regulation of CXCL8 expression and secretion by intestinal epithelial cells in response to P2Y6 receptor activation (39.43)
Bibliographic record
Abstract
Abstract Extracellular nucleotides are recognized as inflammatory-active molecules. We have investigated the role of extracellular uridine 5’-triphosphate (UDP) and its associated P2Y6 receptor in the secretion of CXCL8 by intestinal epithelial cells. We have also characterized signaling pathways linking UDP to the expression of CXCL8. The effect of the P2Y6 activation by UDP on chemokine expression and release by epithelial cells was determined using combination of western blots, luciferase assays, Q-PCR and ELISA. We have monitored the activation of different signaling molecules by western blots, luciferase assays and EMSA, along with different pharmacological inhibitors. Stimulation of Caco-2 cells with UDP lead to PKCδ and PKCζ, as well as ERK 1/2 and NFκB-p65 phosphorylation. Mutations of the NFκB binding site in the minimal promoter of the human CXCL8 completely abolished the luciferase activity stimulated with UDP. EMSA assays showed the importance of NFκB binding site for the initiation of transcription of CXCL8 by P2Y6. In this study, we showed the most detailed signaling pathway known so far for P2Y6 receptor its implication in UDP stimulate CXCL8 expression and release by intestinal epithelial cells.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".