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Id3 is a critical regulator of γδ-T cell development (153.41)

2011· article· en· W4313349880 on OpenAlexaff
Sang Yun Lee, Juan Carlos Zúñiga‐Pflücker, David L. Wiest

Bibliographic record

VenueThe Journal of Immunology · 2011
Typearticle
Languageen
FieldImmunology and Microbiology
TopicImmune Cell Function and Interaction
Canadian institutionsUniversity of Toronto
Fundersnot available
KeywordsBiologyT-cell receptorCell biologyT cellRegulatorNegative selectionCell fate determinationRepertoireLineage (genetic)ImmunologyGeneticsGeneTranscription factorImmune system

Abstract

fetched live from OpenAlex

Abstract αβ and γδ T cells are thought to arise from a common precursor during development in the thymus. Previously, we have shown that αβ/γδ lineage choice is dictated by differences in T cell receptor (TCR) signal strength defined by differential activation of the ERK-Egr-Id3 pathway. In particular, we found that Id3 is required for strong TCR signals to both promote adoption of the γδ-fate and oppose the αβ-fate outcome. Importantly, the effects of Id3-deficiency on γδ T cell development differ depending on the γδ subset. While Id3-deficiency impairs the development of the Vγ2-expressing and Vγ3 dendritic epidermal T cell (DETC) subsets, it results in the dramatic expansion of Vγ1.1Vδ6.3 T cells which are thought to be innate type NK γδ T cells. Fetal thymic organ culture analysis indicates that Id3-deficiency blocks DETC development by interfering with selection in the thymus, not by blocking expression of the canonical DETC TCR complex. Conversely, single-cell PCR analysis has revealed that the junctional diversity of CDR3s of the Vγ1.1Vδ6.3 expressing cells that are expanded in Id3-deficient mice exhibit evidence of ligand-mediated selection. These results are consistent with the notion that development of these cells is normally restricted by ligand-engagement, but this restriction is disabled in the absence of Id3. Altogether, these results indicate that Id3 plays a critical role not only in regulating fate selection, but also in shaping the γδ TCR repertoire.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.011

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.021
GPT teacher head0.234
Teacher spread0.213 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2011
Admission routes1
Has abstractyes

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