The cKrox transgene affects the development and function of multiple T cell types. (64.10)
Bibliographic record
Abstract
Abstract The zinc finger transcription factor cKrox is crucial for CD4 T cell development. Mice lacking a functional cKrox gene lack CD4 T cells. Furthermore, in cKrox-deficient mice, developing T cells expressing a MHC class II-restricted TCR are redirected into the CD8 T cell lineage. We investigated the effect of the cKrox transgene on the development and function of two other types of T cells, namely self-specific CD8 T cells and CD4 T regulatory cells. In TCR transgenic mice that express a male-specific TCR (H-Y TCR), the only CD8 T cells that developed in male TCR transgenic mice are self-specific CD8 T cells. These H-Y TCR+ self-specific CD8 T cells are characterized by high expression of CD44, CD122, Ly6C, 1B11, NK cell markers and proliferative response in response to IL-2 or IL-15. We found that the cKrox transgene converted these self-specific CD8 T cells into CD4 T cells. Furthermore, the converted CD4 T cells lost the characteristics of these self-specific CD8 T cells described above. Notably, the converted self-specific CD8 T cells express genes that are characteristic of CD4 T cells (GATA-3 and IL-2). The numbers of CD4 T regulatory cells were significantly higher in cKrox transgenic mice and these cells expressed higher suppressor activity compared to CD4 T regulatory cells from control mice. These studies indicate that the cKrox transgene affects the development and function of multiple T cell types. This work is supported by the Canadian Cancer Society.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.008 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".