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Regulation of IL-37 and its signaling co-receptor IL-1R8 in HIV infection

2019· article· en· W4313365697 on OpenAlexaff
Ali Ahmad, Suzanne Samarani, Ayoub Abulkhir, Devendra Amre

Bibliographic record

VenueThe Journal of Immunology · 2019
Typearticle
Languageen
FieldImmunology and Microbiology
TopicHIV Research and Treatment
Canadian institutionsUniversité de MontréalCentre Hospitalier Universitaire Sainte-Justine
Fundersnot available
KeywordsCytokineImmune systemCytokine receptorBiologyReceptorImmunologyFlow cytometryPeripheral blood mononuclear cellInterleukin 6In vitroBiochemistry

Abstract

fetched live from OpenAlex

Abstract IL-37 is a member of the IL-1 family with potent anti-inflammatory and immune deviating effects. The cytokine exerts its biological effects via IL-1R8 (also formerly known as TIR-8 and SIGIRR), the signaling component of its receptor. Little is known about the regulation of the cytokine and of its receptor in HIV infection. The objective of this study was to investigate how the production of the cytokine and the expression of the signalling component of its receptor on immune cells was regulated in HIV infection. The study was conducted using biological samples from a cross section of HIV-infected individuals. Concentrations of the cytokine and soluble (shed) IL-1R8 in serum samples were determined by ELISA. The expression of IL-1R8 on immune cells was determined by flow cytometry. The effects of exogenous IL-37 on HIV replication in human PHA blasts were determined in in vitro assays. We found that the cytokine concentrations tended to decrease in treatment-naïve HIV-infected individuals compared with the ART-treated patients. Higher concentrations of the cytokine were observed in sera from LTNP. The transcripts for the most complete isoform of the cytokine, b, disappeared from the patients’ PBMC. The expression of IL-1R8 on immune cells was decreased in HIV-infected individuals. On the other hand, concentrations of its soluble form increased in the sera from the virus-infected individuals. The trend was reversed in the patients undergoing anti-retroviral treatment. Soluble IL-1R8 attenuated anti-inflammatory effects of the cytokine. Collectively, these results suggest that the IL-37/IL-1R8 axis is functionally compromised in HIV-infected individuals.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.259
Teacher spread0.247 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2019
Admission routes1
Has abstractyes

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