A vital control of intracellular <i>Chlamydia</i> infection requires interleukin-17 receptor-mediated signaling (MUC3P.946)
Bibliographic record
Abstract
Abstract IL-17A is a pleiotropic proinflammatory cytokine that has a vital role in host resistance against extracellular bacterial and fungal infections. However, its role in controlling intracellular bacterial infection remains elusive. Here, we examined the IL-17/IL-17 receptor-mediated signaling in controlling intracellular Chlamydia muridarum infection in non-hematopoietic cells in vitro and in vivo. Remarkably, Chlamydia replication in mouse embryonic fibroblasts (MEF) was significantly increased in IL-17RC-knockout (IL-17RCKO) MEF and, to a lesser extent, in IL-17RAKO MEF compared to WT MEF at 6 and 12 hrs post-infection. To verify this finding in vivo, a respiratory Chlamydia infection model was established in bone marrow (BM) chimeric mice using WT BM donor cells transferred into γ-irradiated WT, IL-17RAKO and IL-17RCKO mice. Both IL-17RAKO and IL-17RCKO BM chimeric mice had significantly elevated bacterial burden in the lung compared to WT mice at days 5 and 11 post-infection. However, IL-17RCKO, but not IL-17RAKO, BM chimeric mice exhibited significant body weight loss compared to WT mice during early infection. While IL-17RAKO BM chimeric mice displayed an increased IL-17A response, IL-17RCKO mice showed an increased IFN-γ response in the lung. Our data demonstrates an indispensable role of the IL-17RC-mediated signaling in controlling Chlamydia infection and suggests differential roles of IL-17RA- and IL-17RC-mediated signaling in shaping Chlamydia-induced host responses.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".