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Record W4318016607 · doi:10.1212/nxg.0000000000200048

Novel Homozygous Variant in <i>COQ7</i> in Siblings With Hereditary Motor Neuropathy

2023· article· en· W4318016607 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueNeurology Genetics · 2023
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCoenzyme Q10 studies and effects
Canadian institutionsUniversity of Ottawa
FundersGenome AlbertaCanada Research ChairsGenome British ColumbiaMuscular Dystrophy CanadaCanadian Institutes of Health ResearchGenome CanadaOntario GenomicsOntario Genomics InstituteUniversity of Ottawa
KeywordsExome sequencingMuscle biopsyCompound heterozygosityBiologyCoenzyme Q10CarnitineInternal medicineMethionineMuscle atrophyHydroxylationAtrophyAmino acidEndocrinologyMedicinePathologyGeneticsBiochemistryBiopsyGeneEnzymeMutation

Abstract

fetched live from OpenAlex

<h3>Background and Objectives</h3> Coenzyme Q<sub>10</sub> (CoQ<sub>10</sub>) is an important electron carrier and antioxidant. The COQ7 enzyme catalyzes the hydroxylation of 5-demethoxyubiquinone-10 (DMQ<sub>10</sub>), the second-to-last step in the CoQ<sub>10</sub> biosynthesis pathway. We report a consanguineous family presenting with a hereditary motor neuropathy associated with a homozygous c.1A &gt; G p.? variant of <i>COQ7</i> with abnormal CoQ<sub>10</sub> biosynthesis. <h3>Methods</h3> Affected family members underwent clinical assessments that included nerve conduction testing, histologic analysis, and MRI. Pathogenicity of the <i>COQ7</i> variant was assessed in cultured fibroblasts and skeletal muscle using a combination of immunoblots, respirometry, and quinone analysis. <h3>Results</h3> Three affected siblings, ranging from 12 to 24 years of age, presented with a severe length-dependent motor neuropathy with marked symmetric distal weakness and atrophy with normal sensation. Muscle biopsy of the quadriceps revealed chronic denervation pattern. An MRI examination identified moderate to severe fat infiltration in distal muscles. Exome sequencing demonstrated the homozygous <i>COQ7</i> c.1A &gt; G p.? variant that is expected to bypass the first 38 amino acid residues at the n-terminus, initiating instead with methionine at position 39. This is predicted to cause the loss of the cleavable mitochondrial targeting sequence and 2 additional amino acids, thereby preventing the incorporation and subsequent folding of COQ7 into the inner mitochondrial membrane. Pathogenicity of the <i>COQ7</i> variant was demonstrated by diminished COQ7 and CoQ<sub>10</sub> levels in muscle and fibroblast samples of affected siblings but not in the father, unaffected sibling, or unrelated controls. In addition, fibroblasts from affected siblings had substantial accumulation of DMQ<sub>10</sub>, and maximal mitochondrial respiration was impaired in both fibroblasts and muscle. <h3>Discussion</h3> This report describes a new neurologic phenotype of <i>COQ7</i>-related primary CoQ<sub>10</sub> deficiency. Novel aspects of the phenotype presented by this family include pure distal motor neuropathy involvement, as well as the lack of upper motor neuron features, cognitive delay, or sensory involvement in comparison with cases of <i>COQ7</i>-related CoQ<sub>10</sub> deficiency previously reported in the literature.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.805
Threshold uncertainty score0.871

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.216
Teacher spread0.208 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it