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Record W4318042260 · doi:10.3390/pr11020370

Enzymatic Hydrolysis of Complex Carbohydrates and the Mucus in a Mathematical Model of a Gut Reactor

2023· article· en· W4318042260 on OpenAlexafffund
Thulasi Jegatheesan, Arun S. Moorthy, Hermann J. Eberl

Bibliographic record

VenueProcesses · 2023
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGut microbiota and health
Canadian institutionsUniversity of Guelph
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsMucusCompartment (ship)Substrate (aquarium)MucinHydrolysisChemistryBiochemistryMetabolismMicrobial metabolismBiophysicsBiologyBacteriaEcology

Abstract

fetched live from OpenAlex

The human gut microbiota rely on complex carbohydrates for energy and growth, particularly dietary fiber and host-produced mucins. These complex carbohydrates must first be hydrolysed by certain microbial groups to enable cross-feeding by the gut microbial community. We consider a mathematical model of the enzymatic hydrolysis of complex carbohydrates into monomers by a microbial species. The resulting monomers are subsequently digested by the microbial species for growth. We first consider the microbial species in a single compartment continuous stirred-tank reactor where dietary fiber is the only available substrate. A two compartment configuration in which a side compartment connected by diffusion is also studied. The side compartment is taken to be the mucus layer of the human colon, providing refuge from washout and an additional source of complex carbohydrate in the form of mucins. The two models are studied using stability analysis, numerical exploration, and sensitivity analysis. The delay in substrate availability due to hydrolysis results in bistability and the unconditional asymptotic stability of the trivial equilibrium. The addition of the mucus compartment allows the microbial species to survive under conditions that would otherwise result in washout in a comparable single compartment reactor. This would suggest that depending on the features of the gut microbiota being studied, extracellular hydrolysis and a representation of the mucus layer should be included in mathematical and lab reactor models of the human gut microbiota.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.003
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Simulation or modeling · Consensus signal: Simulation or modeling
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.010
Threshold uncertainty score0.020

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.003
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.002
Bibliometrics0.0010.001
Science and technology studies0.0010.002
Scholarly communication0.0020.001
Open science0.0010.002
Research integrity0.0030.001
Insufficient payload (model declined to judge)0.0040.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.026
GPT teacher head0.275
Teacher spread0.249 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designSimulation or modeling
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2023
Admission routes2
Has abstractyes

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