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Record W4319348988 · doi:10.33612/diss.570000248

Smaller, denser, smarter: improved Strand-seq library preparation, inversion genotyping, and phasing

2023· dissertation· en· W4319348988 on OpenAlexfundno aff
Vincent C. T. Hanlon

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomic variations and chromosomal abnormalities
Canadian institutionsnot available
FundersCanadian Institutes of Health ResearchTerry Fox Research InstituteNederlandse Organisatie voor Wetenschappelijk Onderzoek
KeywordsDNA sequencingGeneticsDNAGenotypingComputational biologyBiologyComputer scienceAlgorithmGeneGenotype

Abstract

fetched live from OpenAlex

Polymorphic inversions are ubiquitous in humans, and they have been linked to both adaptation and disease.Following their discovery in Drosophila more than a century ago, inversions have proved to be more elusive than other structural variants.A wide variety of methods for the detection and genotyping of inversions have recently been developed, including several types of DNA sequencing approaches, optical mapping, and techniques based on selective amplification by PCR.Many of the methods apply complex wet lab protocols or increasingly refined genomic analyses.This review is an attempt to provide a practical summary of the methods that are in current use and to compare their suitability both for genome-wide inversion discovery and for the targeted genotyping of known inversions.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.002
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.010
Threshold uncertainty score0.033

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0020.002
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.001
Science and technology studies0.0010.000
Scholarly communication0.0020.001
Open science0.0010.001
Research integrity0.0010.004
Insufficient payload (model declined to judge)0.0100.011

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.228
Teacher spread0.221 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

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