Cloning and Expression Analysis of <i>GmSAP3</i> Gene in Soybean
Bibliographic record
Abstract
Stress is an important factor that affecting plant growth and crop yield. Stress Associated Protein (SAP), a type of zinc finger protein, is widely involved in plant development and stress response. In order to explore the function of SAP gene in soybean, we cloned the GmSAP3 gene in Shangdou 1201 and carried out bioinformatics analysis, then the expression pattern of GmSAP3 in soybean tissues and temperature stress was analyzed by real-time PCR. The results showed that the CDS of GmSAP3 was 513 bp and encoded 170 amino acids. The isoelectric point (pI) of GmSAP3 was 6.79 and its molecular weight was 18.30568 kD. GmSAP3 contained two conserved domains (zf-A20 and ZnF- AN1), and evolution analysis showed that GmSAP3 is closely related to GmSAP26. RT-PCR results showed that GmSAP3 mainly expressed in roots and leaves, and GmSAP3 is induced by high temperature stress. It is speculated that GmSAP3 play an important role in soybean stress response. This study may provide theoretical foundation for further research on the function of GmSAP3.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".