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Record W4321077501 · doi:10.1038/s41587-023-01657-3

TACCO unifies annotation transfer and decomposition of cell identities for single-cell and spatial omics

2023· article· en· W4321077501 on OpenAlexfundno aff
Simon Mages, Noa Moriel, Inbal Avraham‐Davidi, Evan Murray, Jan Watter, Fei Chen, Orit Rozenblatt–Rosen, Johanna Klughammer, Aviv Regev, Mor Nitzan

Bibliographic record

VenueNature Biotechnology · 2023
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSingle-cell and spatial transcriptomics
Canadian institutionsnot available
FundersNational Institute of Diabetes and Digestive and Kidney DiseasesOffice of Extramural Research, National Institutes of HealthNational Cancer InstituteNational Human Genome Research InstituteCouncil for Higher EducationHebrew University of JerusalemLudwig Center at HarvardDeutsche ForschungsgemeinschaftNational Institutes of HealthU.S. Department of Health and Human ServicesNational Institute of Mental HealthHuman Frontier Science ProgramEuropean CommissionKlarman Cell Observatory, Broad InstituteGoogleAzrieli FoundationIsrael Science FoundationNational Institute of Allergy and Infectious DiseasesHoward Hughes Medical Institute
KeywordsAnnotationDECIPHERComputer scienceDropout (neural networks)Matching (statistics)Variety (cybernetics)Transfer (computing)Data miningComputational biologyBiological systemArtificial intelligenceMachine learningBiologyBioinformaticsMathematics

Abstract

fetched live from OpenAlex

Transferring annotations of single-cell-, spatial- and multi-omics data is often challenging owing both to technical limitations, such as low spatial resolution or high dropout fraction, and to biological variations, such as continuous spectra of cell states. Based on the concept that these data are often best described as continuous mixtures of cells or molecules, we present a computational framework for the transfer of annotations to cells and their combinations (TACCO), which consists of an optimal transport model extended with different wrappers to annotate a wide variety of data. We apply TACCO to identify cell types and states, decipher spatiomolecular tissue structure at the cell and molecular level and resolve differentiation trajectories using synthetic and biological datasets. While matching or exceeding the accuracy of specialized tools for the individual tasks, TACCO reduces the computational requirements by up to an order of magnitude and scales to larger datasets (for example, considering the runtime of annotation transfer for 1 M simulated dropout observations).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.010
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.007
Threshold uncertainty score0.017

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0030.010
Meta-epidemiology (narrow)0.0020.001
Meta-epidemiology (broad)0.0020.003
Bibliometrics0.0020.002
Science and technology studies0.0010.002
Scholarly communication0.0030.003
Open science0.0030.005
Research integrity0.0030.003
Insufficient payload (model declined to judge)0.0030.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.232
Teacher spread0.225 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations83
Published2023
Admission routes1
Has abstractyes

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