Effects of Controlled Culture Conditions on Chemical Composition and Antimicrobial Activities of Mentha rotundifolia Essential Oils
Bibliographic record
Abstract
The study objectives were the determination of Mentha rotundifolia essential oils composition using GC/MS analysis and the evaluation of their antimicrobial activities. In addition, the determination of the relationships between plants acclimatization conditions, essential oils composition and antimicrobial activities. Essential oils extracted via hydrodistillation method from wild plants and acclimatized plants in different culture conditions of Mentha rotundifolia. Five selected pathogenic microbial strains were used to evaluate EOs in vitro antimicrobial activities. Essential oils GC/MS analysis revealed the dominance of the oxygenated monoterpene (piperitenone oxide). A significant effect of plant culture conditions in acclimatization room on essential oils composition compared to the control was observed. We also noted that antimicrobial activities of extracted essential oils from acclimatized plants were higher than those from field-grown M. rotundifolia plants. Content of Piperitenone Oxide in acclimatized plantlets is 93.07% for plantlets cultured at 16°C and a photoperiod of 16 hours light / 8 hours dark, statistically higher than wild plants where it is around 78%. We also noted the presence of Limonene (5.7%) in plantlets grown at 35°C which is significantly different than that of the wild plants (1.55 %). Essential oils antimicrobial activities showed that the plantlets grown at 16 °C or in total darkness were more active towards the tested strains. While, the EO of plantlets cultured at 35 °C were the least active.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".