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Record W4324295543 · doi:10.1117/12.2649196

Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

2023· article· en· W4324295543 on OpenAlexaff
Wentao Wu, Christoph Brandt, Shuo Tang

Bibliographic record

Venuenot available
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicAdvanced Fluorescence Microscopy Techniques
Canadian institutionsUniversity of British Columbia
Fundersnot available
KeywordsTwo-photon excitation microscopyMicroscopyPhotonicsPhotonSecond-harmonic generationOpticsSIGNAL (programming language)High harmonic generationExcitationPhysicsFluorescence microscopeMaterials scienceFluorescenceOptoelectronicsLaserComputer science

Abstract

fetched live from OpenAlex

We report on a simultaneous 2-photon and 3-photon signal acquisition method for label-free multiphoton microscopy. With dual excitation wavelengths of 1580 nm and 790 nm and a set of filter designs, multiple contrasts including second harmonic generation, third harmonic generation, and two-photon-excitation-fluorescence images are detected and separated. The spectrum of the nonlinear signals is measured and verified by a spectrometer. Depth-resolved multimodal images are demonstrated on a silicon photonic chip and leaf samples. The simultaneous 2- and 3-photon microscopy is shown to have great potential for label-free in-vivo imaging.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.019
Threshold uncertainty score0.947

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.296
Teacher spread0.289 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

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