Plasticity of host cell organelles in response to Pb infection
Bibliographic record
Abstract
Abstract Plasmodiophora brassicae ( Pb ) is the most important member of the plant pathogenic protist subgroup, Rhizaria. Pb is an obligate soil-borne plant root pathogen which makes the study of Pb biology and its interaction with its host challenging. To overcome this challenge, we adopted an axenic cell culture method to study the interaction of Pb with its host plant Brassica spp . at the cellular and molecular levels. Pb , during its life cycle within the host, was confined to the cytoplasm of the infected cells. Our results showed that upon Pb infection, the dynamics of the plant cell organelles including mitochondria, plastids, nuclei, vacuoles, vesicles were altered. Both Plant’s and parasite’s diacylglycerol acyltransferases ( DGAT ) genes were highly expressed in the calli, which indicated a need for the oil accumulation at the switch between replication and growth stages of the pathogen. This notion was supported by the accumulation of oil bodies that were only observed inside the pathogen cells. Our results also indicated that starch was utilized by the Pb in infected cells, an indication that Pb induces cell viability by altering the host carbon metabolism. The ongoing dynamic changes in the infected plant cell organelles suggest that plant responds to Pb infection, through the induction of organellar plasticity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".