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Record W4377821134 · doi:10.1002/chem.202301566

Cover Feature: In Vitro Selection and Characterization of a DNAzyme Probe for Diverse Pathogenic Strains of <i>Clostridium difficile</i> (Chem. Eur. J. 36/2023)

2023· article· en· W4377821134 on OpenAlexaff
Shuwen Qian, Dingran Chang, Jimmy Gu, Bruno J. Salena, Yingfu Li

Bibliographic record

VenueChemistry - A European Journal · 2023
Typearticle
Languageen
FieldMedicine
TopicClostridium difficile and Clostridium perfringens research
Canadian institutionsMcMaster University
Fundersnot available
KeywordsDeoxyribozymeClostridium difficileIn vitroClostridiumCleavage (geology)MicrobiologyChemistryBiologyComputational biologyBiochemistryBacteriaGeneticsAntibioticsDNA

Abstract

fetched live from OpenAlex

An RNA-cleavage fluorogenic DNAzyme has been made for recognizing diverse strains of Clostridium difficile that frequently cause an infectious disease known as C. difficile infection (CDI). Upon encountering C. difficile cells, this DNAzyme cuts itself to produce a fluorescent signal, thus making it useful as a molecular probe for CDI diagnosis. This DNAzyme is highly specific as it stays silent in the presence of several control bacterial cells. The DNAzyme has an intriguing secondary structure with a pseudoknot arrangement that is essential for its functionality. More information can be found in the Research Article by Y. Li and co-workers (DOI: 10.1002/chem.202300240).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.075
Threshold uncertainty score0.250

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0750.028

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.262
Teacher spread0.239 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

Explore more

Same venueChemistry - A European Journal→Same topicClostridium difficile and Clostridium perfringens research→French-language works237,207→