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Record W4384211018 · doi:10.1039/d3cp02661b

Protomers of the green and cyan fluorescent protein chromophores investigated using action spectroscopy

2023· article· en· W4384211018 on OpenAlexafffund
Eleanor K. Ashworth, Jordan Dezalay, Christopher R. M. Ryan, Christian Ieritano, W. Scott Hopkins, Isabelle Chambrier, Andrew N. Cammidge, Mark H. Stockett, Jennifer A. Noble, James N. Bull

Bibliographic record

VenuePhysical Chemistry Chemical Physics · 2023
Typearticle
Languageen
FieldNeuroscience
TopicPhotoreceptor and optogenetics research
Canadian institutionsUniversity of Waterloo
FundersNatural Sciences and Engineering Research Council of CanadaEngineering and Physical Sciences Research CouncilMitacsCentre National d’Etudes SpatialesCentre National de la Recherche ScientifiqueSwedish Foundation for International Cooperation in Research and Higher EducationAgence Nationale de la RechercheInstitut national des sciences de l'UniversUniversity of East Anglia
KeywordsCyanChromophoreFluorescenceSpectroscopyChemistryFluorescence spectroscopyGreen fluorescent proteinFluorescent proteinFörster resonance energy transferPhotochemistryAction (physics)Yellow fluorescent proteinBiophysicsBiochemistryPhysicsBiologyOptics

Abstract

fetched live from OpenAlex

, with the main band for the carbonyl protomer red-shifted by ≈60 nm relative to the lower-energy imine protomer. The present strategy for investigating protomers can be applied to a wide range of other biochromophore ions.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.021
Threshold uncertainty score0.616

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.076
GPT teacher head0.335
Teacher spread0.259 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations5
Published2023
Admission routes2
Has abstractyes

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