Cloning and Expression Pattern Analysis of Flowering Related Gene
Bibliographic record
Abstract
A core fragment highly homologous with VERNALIZATION INSENSITIVE 3(VIN3) was obtained on the basis of preliminary Agapanthus praecox ssp.Orientalis transcriptome sequencing results.The full length sequence of cDNA, VIN3 gene of A. praecox was gained by the RACE method, which was named ApVIN3 with the accession number of KJ472789.The results of sequence analysis showed that the ApVIN3 gene length of cDNA was 1 943 bp, which contained an open reading frame of 1 680 bp, 559 encoding amino acids, untranslated region (UTR) 5' and 3' with the length of 88 bp and 175 bp, respectively.ApVIN3 encoding is a PHD-finger protein with a conserved sequence structure of C4HC3 (cys4-His-cys3), which is highly similar to the VIN3 protein of Brachypodium distachyum (L.) Beauv (XP_003573553.1).Quantitative Real-time PCR show: The regulation of gene expression in quantity by temperature in Agapanthus africanus Hoffmgg.shoot apex, with prolonging the time of low temperature rising.This gene may play an important role in the process of brucella induction, and its function and regulation mechanism can be studied deeply.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".