Transgene silencing in the moss, Physcomitrella (<i>Physcomitrium patens</i>)
Bibliographic record
Abstract
Gene targeting is a powerful tool for functional genetic analysis. It has proved especially effective in the model plant, Physcomitrella ( Physcomitrium patens (Hedw.) Mitt.), in which integration of exogenous DNA into the genome occurs with very high efficiency at targeted positions by homologous recombination. Here, we present a preliminary characterisation of a poorly documented property of Physcomitrella targeted gene knockout lines, namely, silencing of reporter and selective marker genes of integrated targeting constructs. We discovered a high incidence of transgene silencing among Physcomitrella knockout lines and, as is the case in seed plants, cytosine (DNA) methylation of the transgenes is correlated with their silencing. Silencing in Physcomitrella is characterised by metastable epigenetic inheritance through repetitive mitosis and variable stability through meiosis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".