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Record W4385499564 · doi:10.5376/be.2023.13.0001

Analysis of ATH1 Transcription Activity and Co-IP of ATH1 Protein Interaction with OFP1

2023· article· en· W4385499564 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

venuePublished in a venue whose home country is Canada.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueBiological Evidence · 2023
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPhotosynthetic Processes and Mechanisms
Canadian institutionsnot available
FundersHeilongjiang Academy of Agricultural SciencesNatural Science Foundation of Heilongjiang Province
KeywordsTranscription factorTranscription (linguistics)BiotechnologyCell biologyGeneChemistryBiologyBiochemistry

Abstract

fetched live from OpenAlex

ATH1 has multifaceted functions in regulating growth and development, and possibly regulate development by interacting with OFP1 protein. However, the protein interaction of ATH1 with OFP1 has not been further confirmed, and the transcription activity of ATH1 has not been determined. In this work, the interaction ATH1 with OFP1 was further confirmed by using Co-IP, and the transcription activity of ATH1 was determined by using Protoplast transfection system. The results showed that the ATH1-OFP1 protein complexes could be formed in   vivo , and ATH1 was a transcription inhibitory factor, and OFP1 could enhance the transcription inhibitory activity of ATH1. This study will provide molecular basis for analysis of the regulatory functions of OFP-TALE complexes in plant development, which is great significance for improving important plant traits.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.017
Threshold uncertainty score0.237

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.055
GPT teacher head0.317
Teacher spread0.262 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it