P1260: BIODISTRIBUTION OF LABELED AUTOLOGOUS AND ALLOGENEIC BONE MARROW MESENCHYMAL STROMAL CELLS IN RABBITS AFTER INTRAVENOUS ADMINISTRATION
Bibliographic record
Abstract
Topic: 21. Stem cell transplantation - Experimental Background: Mesenchymal stromal cells (MSC) are used for cell therapy. This type of cells has the ability to immunomodulate and migrate to inflamed tissue areas. Studies of the biodistribution of MSCs in animals have shown equivocal results. Depending on the route of administration and the source of origin, MSCs are mainly found in the lungs, liver and other organs, but also in inflammatory foci. Fluorescent dyes can be used to study the pharmacokinetics of MSCs. Carboxyfluorescein succinimidyl ether (CFSE) stably binds to cytoplasmic proteins and persists in the cell for up to 8 division cycles. Aims: To study the biodistribution of CFSE-labeled bone marrow MSCs after intravenous injection in rabbits Methods: Our experiment was performed with mature rabbits, not albinos, of both sexes, weighing 3000-4000 g. The source of MSC was a rabbits bone marrow. Cultivation was carried out in an atmosphere of 5% CO2 at a temperature of 37°C on αMEM medium (StemCells, Canada) containing platelet-rich plasma (4%), heparin (Russia, 2 U/mL), L-glutamine (StemCells, Canada, 2 mM). Cells were detached from plastic with 0.25% trypsin and stained with CFSE (Sigma, USA). The labeled cells were injected into the rabbit ear vein at an amount of (1,0-1,5)×106/kg. Animals were removed from the experiment through 1 (n=3) and 24 (n=3) hours after administration of allogeneic cells, and 24 hours (n=2) after the injection of autologous cells. The heart, lungs, liver, spleen, kidneys, and femurs were taken, followed by the histological preparations in the conventional way. After staining with 4’,6-diamidino-2-phenylindole, tissue sections were examined with an Axio Scope A1 luminescent microscope (×1000, excitation wavelength 475 nm, emission wavelength 530 nm). Count the number of labeled cells in the field of view. At least 10 fields per sample were evaluated. The results are presented as mean, minimum and maximum values. Results: At 1 hour after the allogeneic cells injection, the largest number of labeled MSCs in 1 field of view was detected in the bone marrow (1.7 (0-4)) and liver (1.2 (0-4)). Single luminescent cells were noted in the spleen (0.7 (0-2)) and lungs (0.4 (0-2)). There were no MSCs in the heart and kidneys. The mean number of labeled allogeneic MSCs in 1 field was 0.2 (0-3) in the kidney and 0.3 (0-1) in the bone marrow over a 24-hour period. Labeled cells were not found in the heart, lungs and spleen. Twenty-four hours after administration of autologously labeled MSCs, the mean number of luminescent cells in the bone marrow was 3.4 (0-9). Single CFSE+ cells were detected in liver (0.5 (0-2)), kidney (0.3 (0-2)) and lung (0.1 (0-1)). MSCs with specific green light were not found in the heart and spleen. Summary/Conclusion: The selective accumulation of CFSE+ MSCs in the bone marrow and liver 1 h after injection confirms their ability to homing. A decrease in the number of fluorescent allogeneic MSCs in the recipient’s body after 24 hours may be associated with immune rejection and indicates the absence of immune privilege of the studied cells. Labeled autologous MSCs remained in the accumulation organs after 24 h, which confirms the participation of histocompatibility antigens in the elimination of MSC from the organism. Keywords: Bone Marrow, Mesenchymal cells, Autologous, Allogeneic
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".