Culture-based Determination of Verticillium Densities in Soil Overestimates Disease Pressure of Verticillium Wilt of Potato in Manitoba
Bibliographic record
Abstract
Abstract Verticillium wilt affects potato fields in Manitoba where 80% of the fields are planted to the moderately susceptible cultivar, Russet Burbank. An accurate determination of Verticillium dahliae inoculum in soil is critical for disease management. In this study, we investigated the presence of microsclerotia-producing Verticillium species in potato fields in Manitoba and compared published quantitative real-time PCR assays for V. dahliae, V. tricorpus and V. longisporum against a traditional plating method. Selected real-time PCR assays could differentiate and quantify the major microsclerotia-producing species, V. dahliae , V. tricorpus and V. longisporum . Results showed that the presence of V. tricorpus caused an overestimation of V. dahliae propagule density when using the plating method. As a result, Verticillium wilt severity was negatively related to cfu from traditional plating, while positive with the amounts of V. dahliae genomic DNA in soils.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".