MétaCan
Menu
Back to cohort

Qiagen RNEasy PowerMicrobiome RNA extraction kit v2

2023· preprint· en· W4386275979 on OpenAlexaff
Michael Dan Siemon

Bibliographic record

Venuenot available
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMolecular Biology Techniques and Applications
Canadian institutionsWestern University
Fundersnot available
KeywordsIsoamyl alcoholChromatographyCentrifugationRNAElutionChemistryExtraction (chemistry)Magnetic beadBeadRNA extractionDNAChloroformMaterials scienceAlcoholBiochemistryGene

Abstract

fetched live from OpenAlex

The samples were processed using the Qiagen RNeasy PowerMicrobiome kit with the modifications described by Schang et al., 2021. As a substitute for vortexing described in the kit protocol, bead-beating was used. 100ul of phenol-chloroform-isoamyl alcohol was added to the bead beating tubes before the addition of the membranes. Bead-beating was conducted 4x for 30s at 4 m/s. After pelletizing the samples with centrifugation, the supernatant was processed through spin columns. Spin columns were incubated with DNase Digestion Solution for 15 minutes to isolate only RNA. The RNA was eluted from the spin columns by passing 50 uL of DEPC water through twice.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.004
metaresearch head score (Gemma)0.004
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.073
Threshold uncertainty score0.245

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0040.004
Meta-epidemiology (narrow)0.0060.003
Meta-epidemiology (broad)0.0090.004
Bibliometrics0.0060.004
Science and technology studies0.0040.003
Scholarly communication0.0030.001
Open science0.0060.004
Research integrity0.0020.008
Insufficient payload (model declined to judge)0.0730.066

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.024
GPT teacher head0.326
Teacher spread0.302 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2023
Admission routes1
Has abstractyes

Explore more

Same topicMolecular Biology Techniques and ApplicationsFrench-language works237,207