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Record W4386278264 · doi:10.1093/plcell/koad228

MYB24 orchestrates terpene and flavonol metabolism as light responses to anthocyanin depletion in variegated grape berries

2023· article· en· W4386278264 on OpenAlexaff
Chen Zhang, Zhanwu Dai, Thilia Ferrier, Luis Orduña, Antonio Santiago, Arnau Peris, Darren C. J. Wong, Christian Kappel, Stefania Savoi, Rodrigo Loyola, Alessandra Amato, Bartosz Kozak, Miaomiao Li, Akun Liang, David Carrasco, Carlos Meyer‐Regueiro, Carmen Espinoza, Ghislaine Hilbert, Rosa Figueroa‐Balderas, Dario Cantù, Rosa Arroyo-García, Patricio Arce‐Johnson, Patricia Claudel, Daniel Errandonea, Manuel Rodrı́guez-Concepción, Éric Duchêne, Shao‐shan Carol Huang, Simone D. Castellarin, Giovanni Battista Tornielli, François Barrieu, José Tomás Matus

Bibliographic record

VenueThe Plant Cell · 2023
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant biochemistry and biosynthesis
Canadian institutionsOkanagan University CollegeUniversity of British Columbia, Okanagan CampusUniversity of British Columbia
FundersEuropean Regional Development FundAgencia Estatal de InvestigaciónInstitut National de Recherche pour l'Agriculture, l'Alimentation et l'EnvironnementMinisterio de Ciencia e InnovaciónUniversità degli Studi di VeronaChina Scholarship CouncilEuropean CommissionNational Science Foundation
KeywordsFlavonolsBiologyCarotenoidAnthocyaninPhotoprotectionBerryTerpeneRipeningBotanyPhenylpropanoidChalcone synthaseVeraisonBiochemistryGeneGene expressionPhotosynthesisFlavonoidBiosynthesisAntioxidant

Abstract

fetched live from OpenAlex

Variegation is a rare type of mosaicism not fully studied in plants, especially fruits. We examined red and white sections of grape (Vitis vinifera cv. 'Béquignol') variegated berries and found that accumulation of products from branches of the phenylpropanoid and isoprenoid pathways showed an opposite tendency. Light-responsive flavonol and monoterpene levels increased in anthocyanin-depleted areas in correlation with increasing MYB24 expression. Cistrome analysis suggested that MYB24 binds to the promoters of 22 terpene synthase (TPS) genes, as well as 32 photosynthesis/light-related genes, including carotenoid pathway members, the flavonol regulator HY5 HOMOLOGUE (HYH), and other radiation response genes. Indeed, TPS35, TPS09, the carotenoid isomerase gene CRTISO2, and HYH were activated in the presence of MYB24 and MYC2. We suggest that MYB24 modulates ultraviolet and high-intensity visible light stress responses that include terpene and flavonol synthesis and potentially affects carotenoids. The MYB24 regulatory network is developmentally triggered after the onset of berry ripening, while the absence of anthocyanin sunscreens accelerates its activation, likely in a dose-dependent manner due to increased radiation exposure. Anthocyanins and flavonols in variegated berry skins act as effective sunscreens but for different wavelength ranges. The expression patterns of stress marker genes in red and white sections of 'Béquignol' berries strongly suggest that MYB24 promotes light stress amelioration but only partly succeeds during late ripening.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.025
Threshold uncertainty score0.518

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.221
Teacher spread0.209 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations51
Published2023
Admission routes1
Has abstractyes

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