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Record W4386372633 · doi:10.19044/esj.2023.v19n24p1

First Isolation and Identifiaction of Agriphages in Vegetable Crops in West Africa (Cȏte d’Ivorie): Potential Uses of Biocontrol in Plants

2023· article· en· W4386372633 on OpenAlexfundno aff
Akou Emmanuella Adioumani, Solange Kakou-Ngazoa, Noel David Trebissou Jonhson, Martin Thibaut, Addabah Audrey, Koudu Aristide, Sina K. Mireille, Serge Aoussi, Mireille Dosso

Bibliographic record

VenueEuropean Scientific Journal ESJ · 2023
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicLegume Nitrogen Fixing Symbiosis
Canadian institutionsnot available
FundersUniversité Laval
KeywordsBiologyRAPDRalstonia solanacearumBiological pest controlBiopesticideCropIsolation (microbiology)BacteriaXanthomonasMicrobiologyHorticultureBotanyAgronomyPathogenGenetic diversityPesticide

Abstract

fetched live from OpenAlex

Agriphages or bacterial viruses are ubiquitous in the environment. The discovery of virulent phages against phytobacteria improves crop growth and proposes biopesticide uses for plant diseases. In Africa, many phytobacteria such as Ralstonia, Clavibacter, and Xanthomonas were reported in several regions. This paper focuses on evaluating the presence of agriphages for the biocontrol of phytobacteria in Côte d'Ivoire. Leaves and soil samples were collected from healthy and diseased plants in three sites located in Anyama, Abidjan, and Bingerville. The pretreatments occurred with sterile and physiological water for leaves and soil samples, respectively. The isolation of agriphages was done on specific media with Xanthomonas campestris as bacterial host. Lytic activity was tested on agar media for five bacteria strains. After DNA extraction using the Qiagen method kit, molecular confirmation of agriphages was done by Random Amplified Polymorphic DNA-PCR. From this study, five (5) agriphages were isolated in soil and leaves in site 2. These agriphages have all been isolated from Xanthomonas campestris and have a broad spectrum of lytic activity. Molecular characterization by RAPD-PCR showed that three of these agriphages are DNA phages. The dendrogram showed that phages ΦXanS1 and ΦXanS2 have 93% similarities, while ΦXanS1 and ΦXanS2 are 62% similar to ΦXanF1. This study is the first reported agriphages in West Africa, alongside their potential uses against phytobacteria for biocontrol infection in crops.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.859
Threshold uncertainty score0.227

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.208
Teacher spread0.191 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

Explore more

Same venueEuropean Scientific Journal ESJSame topicLegume Nitrogen Fixing SymbiosisFrench-language works237,207