Effects of Cytokine-Induced killer cells on concanavalin-a induced hepatitis in mouse
Bibliographic record
Abstract
Autoimmune hepatitis (AIH) is a chronic inflammatory disease of liver that the pathogenic mechanisms of AIH have not yet been clarified. All patients with AIH lead to cirrhosis and liver cancer. Presently the best feasible medicines of AIH need aid immunosuppressive medications and liver transplantation which have many side effects, high cost and sustained remission. Thus, immunotherapy is an alternative therapy in which cellular material is injected into a patient. Many previous studies showed cytokine induced killer (CIK) cells, T lymphocytes that have a phenotype of NK cells, have a potential to against several diseases associated with liver. The current research aims to examine protective effects of CIK cells on Concanavalin A (Con A) induced AIH in mice. In this study we use Con A because it is a generally used model for AIH in mice, the activation and recruitment of T cells to the liver. CIK cells cultured from thymus were then used to develope a protocol for generating CIK cells by adding interferon gamma (IFN-g), monoclonal antibody (mAb) against CD3 and interleukin-2 (IL-2). In this study markers show NK1.1 41.60 %, IFN-g 8.56 %, Granzyme 53.40 % and Perforin 8.31 %. Twenty-four hours before Con A injection mice were injected with CIK cells through IV (10 x 10⁶ cells per 200 µl). Mice were intravenously (IV) injected through lateral tail vein for AIH induction (25mg/kg). Lastly mice were euthanized and organs (blood and liver) were collected for biochemical test (AST, ALT) and histopathology study. In histopathology study show significant difference between control group and experimental group, hepatocellular swelling, scattered area of leukocytic infiltration, multiple necrotic area, central veins are grossly intact and no fibrotic tissue. For biochemical test we found that the average of AST level in three group are 48.33, 398.75 and 487.33 U/ml respectively while the average of AST level are 38.33, 113.75 and 199 U/ml respectively. Statistical analysis by SPSS program were used to compare between 2 groups (ConA and ConA & CIK cells group) in pathological study and biochemical test (P<0.05). In this study concluded that CIK cells does not show protective effect on Concanavalin-A indued hepatitis in mouse.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.003 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".