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Record W4387004881 · doi:10.1016/j.isci.2023.108050

Pre- and postsynaptic N-methyl-D-aspartate receptors are required for sequential printing of fear memory engrams

2023· article· en· W4387004881 on OpenAlexaff
Ilaria Bertocchi, Florbela Rocha-Almeida, María Teresa Romero-Barragán, Marco Cambiaghi, Alejandro Carretero‐Guillén, Paolo Botta, Godwin Dogbevia, Mario Treviño, Paolo Mele, Alessandra Oberto, Matthew E. Larkum, Agnès Gruart, Rolf Sprengel, José M. Delgado‐García, Mazahir T. Hasan

Bibliographic record

VenueiScience · 2023
Typearticle
Languageen
FieldNeuroscience
TopicMemory and Neural Mechanisms
Canadian institutionsHealth Canada
FundersAgencia Estatal de InvestigaciónIngeborg Ständer-StiftungNational Institutes of HealthJunta de AndalucíaFundación Tatiana Pérez de Guzmán el BuenoEuropean Regional Development FundMax-Planck-GesellschaftMinisterio de Ciencia e InnovaciónFritz Thyssen Stiftung
KeywordsEngramNeuroscienceOptogeneticsMemory consolidationFear conditioningPrefrontal cortexBasolateral amygdalaAmygdalaPostsynaptic potentialPsychologySynaptic plasticityHippocampusBiologyReceptorCognition

Abstract

fetched live from OpenAlex

The organization of fear memory involves the participation of multiple brain regions. However, it is largely unknown how fear memory is formed, which circuit pathways are used for "printing" memory engrams across brain regions, and the role of identified brain circuits in memory retrieval. With advanced genetic methods, we combinatorially blocked presynaptic output and manipulated N-methyl-D-aspartate receptor (NMDAR) in the basolateral amygdala (BLA) and medial prefrontal cortex (mPFC) before and after cued fear conditioning. Further, we tagged fear-activated neurons during associative learning for optogenetic memory recall. We found that presynaptic mPFC and postsynaptic BLA NMDARs are required for fear memory formation, but not expression. Our results provide strong evidence that NMDAR-dependent synaptic plasticity drives multi-trace systems consolidation for the sequential printing of fear memory engrams from BLA to mPFC and, subsequently, to the other regions, for flexible memory retrieval.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.002
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.006
Threshold uncertainty score0.494

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.002
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.107
GPT teacher head0.337
Teacher spread0.230 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations7
Published2023
Admission routes1
Has abstractyes

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