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Record W4387016655 · doi:10.32920/24192183.v1

Effects of lithium chloride on chondrocyte primary cilia in 3D culture

2023· preprint· en· W4387016655 on OpenAlexaff
Arianna Soave

Bibliographic record

Venuenot available
Typepreprint
Languageen
FieldMedicine
TopicOsteoarthritis Treatment and Mechanisms
Canadian institutionsToronto Metropolitan University
Fundersnot available
KeywordsCiliumChondrocyteCartilageLithium chlorideCell biologyChondrogenesisChemistryAnatomyBiology

Abstract

fetched live from OpenAlex

Articular cartilage is unique and distinguishable amongst other tissue types due to its limited self-healing capacity, driving the need to investigate novel methods capable of replacing or repairing damaged or diseased tissue. In order to create tissue engineered constructs to repair cartilage defects, large populations of chondrocytes are needed, thereby requiring the expansion of primary cells. However, routine methods of cell expansion (e.g. monolayer expansion) tend to result in the loss of chondrogenic phenotype and insensitivity to mechanical stimuli. It has been suggested that the chondrocyte’s primary cilium is one factor responsible in transducing mechanical signals between the cell and its surrounding environment. Thus, this work focused on restoring primary cilia (length and incidence) and mechanoreceptive characteristics using lithium chloride (LiCl). Overall, results revealed that primary cilia length and incidence increased with LiCl concentration, pre-culture duration, and passage number. However, the mechanosensitivity of chondrocytes, as determined by changes in cartilaginous matrix synthesis after exposure to mechanical stimuli, appeared to be dependent on the degree of prior cell passaging. Thus, these results suggest that although the primary cilium acts as a transducer of mechanical signals, the effects of passaging might impact its functionality.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.009
Threshold uncertainty score0.019

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0000.000
Science and technology studies0.0010.000
Scholarly communication0.0010.000
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.259
Teacher spread0.245 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2023
Admission routes1
Has abstractyes

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