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Record W4387675283 · doi:10.1016/j.celrep.2023.113256

Differential DNA damage repair and PARP inhibitor vulnerability of the mammary epithelial lineages

2023· article· en· W4387675283 on OpenAlexafffund
Hye‐Yeon Kim, Kazeera Aliar, Pirashaanthy Tharmapalan, Curtis W. McCloskey, Abhijith Kuttanamkuzhi, Barbara T. Grünwald, Luís Palomero, Mathepan Mahendralingam, Matthew Waas, Arvind Singh Mer, Mitchell J. Elliott, Bowen Zhang, Khalid N. Al‐Zahrani, Ellen Langille, Michael T. Parsons, Swami Narala, Stefan O.P. Hofer, Paul Waterhouse, Razqallah Hakem, Benjamin Haibe‐Kains, Thomas Kislinger, Daniel Schramek, David W. Cescon, Miguel Ángel Pujana, Hal K. Berman, Rama Khokha

Bibliographic record

VenueCell Reports · 2023
Typearticle
Languageen
FieldMedicine
TopicPARP inhibition in cancer therapy
Canadian institutionsLunenfeld-Tanenbaum Research InstituteMount Sinai HospitalUniversity of OttawaPrincess Margaret Cancer CentreUniversity of TorontoUniversity Health Network
FundersCanadian Institutes of Health ResearchTerry Fox Research InstituteCanadian Cancer SocietyUniversity Health Network
KeywordsDNA damagePoly ADP ribose polymerasePARP inhibitorVulnerability (computing)DNA repairBiologyCell biologyDNADifferential (mechanical device)GeneticsCancer researchComputer scienceComputer securityPolymerase

Abstract

fetched live from OpenAlex

It is widely assumed that all normal somatic cells can equally perform homologous recombination (HR) and non-homologous end joining in the DNA damage response (DDR). Here, we show that the DDR in normal mammary gland inherently depends on the epithelial cell lineage identity. Bioinformatics, post-irradiation DNA damage repair kinetics, and clonogenic assays demonstrated luminal lineage exhibiting a more pronounced DDR and HR repair compared to the basal lineage. Consequently, basal progenitors were far more sensitive to poly(ADP-ribose) polymerase inhibitors (PARPis) in both mouse and human mammary epithelium. Furthermore, PARPi sensitivity of murine and human breast cancer cell lines as well as patient-derived xenografts correlated with their molecular resemblance to the mammary progenitor lineages. Thus, mammary epithelial cells are intrinsically divergent in their DNA damage repair capacity and PARPi vulnerability, potentially influencing the clinical utility of this targeted therapy.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.277
Teacher spread0.259 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations6
Published2023
Admission routes2
Has abstractyes

Explore more

Same venueCell ReportsSame topicPARP inhibition in cancer therapyFrench-language works237,207