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Record W4387987363 · doi:10.37777/dscs.v24n1-005

Devil's claw has anti-neuroinflammatory capacity through mitochondrial recovering in microglia cells exposed to rotenone

2023· article· en· W4387987363 on OpenAlexaff
Bianca Fagan Bissacotti, Lauren Pappis, Maiara Callegaro Velho, Tuyla Fontana, Amanda Kolinski Machado, Gabriela Geraldo Sangoi, Erika Leigh Beroncal, Larissa Finger Schaffer, Alencar Kolinski Machado

Bibliographic record

VenueDisciplinarum Scientia. Série Ciências da Saúde · 2023
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPhytochemistry and Biological Activities
Canadian institutionsUniversity of Toronto
Fundersnot available
KeywordsRotenoneMicrogliaViability assayOxidative stressNeuroinflammationNitric oxideReactive oxygen speciesMitochondrionPharmacologyOxidative phosphorylationBiologyChemistryInflammationCellCell biologyBiochemistryImmunologyEndocrinology

Abstract

fetched live from OpenAlex

Neuropsychiatric diseases, such as bipolar disorder, Alzheimer’s, and Parkinson’s disease, are related to mitochondrial dysfunction and chronic inflammatory activation, affecting thousands of people worldwide. Therefore, new alternative therapies with mitochondrial modulation and anti-inflammatory effects, especially involving natural agents are important to search. Harpagophytum procumbens is a notable candidate. It is an African plant with anti-inflammatory action. The objective of this study was to evaluate the anti-neuroinflammatory effect of H. procumbens ethyl acetate fraction in microglia cells. H. procumbens ethyl acetate fraction per se effect was available in SH-SY5Y and BV-2 cells during 24, 48, and 72 hours by cell viability and nitric oxide levels. Only BV-2 cells were exposed to rotenone to induce inflammation-mediated mitochondrial dysfunction and treated with curve concentration of H. procumbens fraction. Cellular proliferation and oxidative metabolism parameters were analyzed. Per se effect results revealed that significative changes are viewer depending on the time of exposure and cell line type. 0.001-400 µg/mL of H. procumbens treatment decreased nitric oxide and reactive oxygen species levels which have been increased by rotenone exposure, furthermore, cell viability recovered after this treatment exposure. H. procumbens was able to act as anti-neuroinflammatory agent, recovering mitochondrial complex I function, reducing oxidative stress, and decreasing cell proliferation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.058
GPT teacher head0.247
Teacher spread0.189 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

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