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Record W4388229104 · doi:10.1101/2023.10.29.564625

Human CD127 negative ILC2s show immunological memory

2023· preprint· en· W4388229104 on OpenAlexaff
Laura Mathä, Lisette Krabbendam, Sergio Martínez-Høyer, Balthasar A. Heesters, Korneliusz Golebski, Chantal M. A. Kradolfer, Maryam Ghaedi, Junjie Ma, Ralph Stadhouders, Claus Bachert, Lars‐Olaf Cardell, Nan Zhang, Gabriële Holtappels, Leanne C. Helgers, Jonathan M. Coquet, Fumio Takei, Hergen Spits, Itziar Martínez-González

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2023
Typepreprint
Languageen
FieldImmunology and Microbiology
TopicIL-33, ST2, and ILC Pathways
Canadian institutionsPrincess Margaret Cancer CentreUniversity Health NetworkTerry Fox Research Institute
FundersKnut och Alice Wallenbergs StiftelseSanofi GenzymeVetenskapsrådetKarolinska InstitutetSanofi
KeywordsInterleukin-7 receptorInnate lymphoid cellImmunologyBiologyImmunityImmune systemT cellMedicineIL-2 receptor

Abstract

fetched live from OpenAlex

SUMMARY Group 2 Innate Lymphoid Cells (ILC2s) serve as key players in type 2 immunity and contribute significantly to maintaining homeostasis and responding to inflammation. Notably, ILC2s are closely implicated in the development of allergic disorders like asthma. While previous research has demonstrated immunological memory in mouse ILC2s, it has remained unclear whether human ILC2s can acquire this form of memory. In this study, we demonstrate the persistence of CD45RO, a marker previously linked to inflammatory ILC2s, in resting ILC2s that have undergone prior activation. These cells concurrently reduce the expression of the canonical ILC marker CD127. Through in vitro experiments involving the isolation and stimulation of CD127-CD45RO+ ILC2s, we observed an augmented ability to produce cytokines and undergo proliferation. CD127-CD45RO+ ILC2s are found in both healthy and inflamed tissues and display a gene signature of cell activation. Memory ILC2s may play a significant role in chronic type 2 diseases, such as allergic asthma and atopic dermatitis. HIGHLIGHTS – Inflamed and healthy tissues contain CD127-ILC2s with a gene signature of cell activation – CD127-CD45RO+ ILC2s from healthy tissues show immunological memory features – Mouse memory ILC2s downregulate CD127 – In vitro stimulation of naïve ILC2s generates memory CD127-CD45RO+ ILC2s

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.018

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0050.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.030
GPT teacher head0.236
Teacher spread0.206 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2023
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)Same topicIL-33, ST2, and ILC PathwaysFrench-language works237,207