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Record W4388680317 · doi:10.1002/pmic.202300395

People with obesity exhibit losses in muscle proteostasis that are partly improved by exercise training

2023· article· en· W4388680317 on OpenAlexaff
Kanchana Srisawat, Connor A. Stead, Katie Hesketh, Mark Pogson, Juliette A. Strauss, Matt Cocks, Ivo Siekmann, Stuart M. Phillips, Paulo Lisböa, Sam O. Shepherd, Jatin G. Burniston

Bibliographic record

VenuePROTEOMICS · 2023
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMuscle metabolism and nutrition
Canadian institutionsMcMaster University
Fundersnot available
KeywordsProteostasisProtein turnoverInternal medicineEndurance trainingEndocrinologyPopulationWastingAerobic exerciseBiologyExercise physiologyMedicineProtein biosynthesisBiochemistry

Abstract

fetched live from OpenAlex

Abstract This pilot experiment examines if a loss in muscle proteostasis occurs in people with obesity and whether endurance exercise positively influences either the abundance profile or turnover rate of proteins in this population. Men with ( n = 3) or without ( n = 4) obesity were recruited and underwent a 14‐d measurement protocol of daily deuterium oxide (D 2 O) consumption and serial biopsies of vastus lateralis muscle. Men with obesity then completed 10‐weeks of high‐intensity interval training (HIIT), encompassing 3 sessions per week of cycle ergometer exercise with 1 min intervals at 100% maximum aerobic power interspersed by 1 min recovery periods. The number of intervals per session progressed from 4 to 8, and during weeks 8–10 the 14‐d measurement protocol was repeated. Proteomic analysis detected 352 differences ( p < 0.05, false discovery rate < 5%) in protein abundance and 19 ( p < 0.05) differences in protein turnover, including components of the ubiquitin‐proteasome system. HIIT altered the abundance of 53 proteins and increased the turnover rate of 22 proteins ( p < 0.05) and tended to benefit proteostasis by increasing muscle protein turnover rates. Obesity and insulin resistance are associated with compromised muscle proteostasis, which may be partially restored by endurance exercise.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.226
Teacher spread0.209 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations11
Published2023
Admission routes1
Has abstractyes

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