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Macrophage Cholesterol Efflux and Atherosclerosis in Psoriasis: A role for microRNA‐33

2017· article· en· W4389021302 on OpenAlexaff
Denuja Karunakaran, Girish Dwevidi, Katey J. Rayner

Bibliographic record

VenueThe FASEB Journal · 2017
Typearticle
Languageen
FieldImmunology and Microbiology
TopicIL-33, ST2, and ILC Pathways
Canadian institutionsUniversity of Ottawa
Fundersnot available
KeywordsMacrophageInflammationABCA1EffluxPsoriasisCholesterolReverse cholesterol transportMedicineImmunologyInternal medicineEndocrinologyChemistryLipoproteinTransporterBiochemistryGene

Abstract

fetched live from OpenAlex

Psoriatic patients have an increased risk in developing atherosclerosis. However, the precise mechanism by which the localized skin inflammation drives atherosclerosis is unclear. Macrophages have been shown to promote localized skin inflammation and also drive atherosclerosis by retaining modified excess cholesterol. Macrophage cholesterol efflux depends on 2 elements: (i) functional circulating HDL interacting with (ii) macrophage ABCA1 cholesterol transporter to release the excess cholesterol into HDL particles to be cleared. microRNA‐33 (miR‐33) levels are markedly elevated in CAD and it directly targets various genes regulating cholesterol transport, mitochondrial function and inflammation to inhibit macrophage cholesterol efflux, promoting atherosclerosis. Thus, novel therapeutics aims to inhibit miR‐33 to treat atherosclerosis. Nonetheless, the mechanism by which macrophage miR‐33 drives atherosclerosis in psoriasis and how psoriasiform‐inflammation accelerates atherosclerosis remains to be elucidated. Here, we aim to determine if psoriasiform macrophages have elevated miR‐33 levels and are defective in mediating cholesterol efflux and thus, accelerating atherosclerosis. Preliminary data indicate that human macrophages treated with key cytokines (e.g. IL‐17A or IL‐23 or TNFα) that drive psoriatic inflammation have increased miR‐33a expression and decreased macrophage cholesterol efflux. Ongoing studies are investigating whether human primary macrophages derived from psoriatic patients have decreased cholesterol efflux relative to control. These studies will provide mechanistic insight as to how miR‐33 is regulated in psoriasiform inflammation and whether anti‐miR33 therapy or other novel therapeutic targeting the macrophage efflux pathways can be used to treat cardiovascular disease in psoriatic patients.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.225
Threshold uncertainty score0.992

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.020
GPT teacher head0.246
Teacher spread0.225 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2017
Admission routes1
Has abstractyes

Explore more

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