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Record W4389040424 · doi:10.1021/acsestwater.3c00288

Factors Affecting the Dynamics of <i>Legionella pneumophila</i>, Nontuberculous Mycobacteria, and Their Host <i>Vermamoeba vermiformis</i> in Premise Plumbing

2023· article· en· W4389040424 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueACS ES&T Water · 2023
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicLegionella and Acanthamoeba research
Canadian institutionsMcGill UniversityPolytechnique Montréal
FundersFonds de recherche du Québec – Nature et technologiesNatural Sciences and Engineering Research Council of Canada
KeywordsLegionella pneumophilaMicrobiologyBiofilmNontuberculous mycobacteriaBiologyLegionellaBacteriaMycobacterium

Abstract

fetched live from OpenAlex

Premise plumbing are complex systems with diverse materials and variable temperature and stagnation time. Opportunistic pathogens are commonly found in premise plumbing, but little is known about the impact of design and operational characteristics of building water systems on their occurrence and survival in water and biofilms. The main objective of this study was to investigate the interplay between material type, temperature, and stagnation on the occurrence and survival of Legionella pneumophila, nontuberculous mycobacteria (NTM), and their host Vermamoeba vermiformis . Twelve CDC biofilm reactors were used to compare concentrations of these microorganisms measured by qPCR in water and biofilms, at 25, 40, 55, or 60 °C, and in contact with six materials: polypropylene (PP), polyvinyl chloride (PVC), EPDM, cross-linked polyethylene (PEX), stainless steel (SS), and copper (Cu). The addition of V. vermiformis induced an average 1.4-log increase in NTM concentrations, while the spiking of two L. pneumophila strains caused a 3-log decrease in NTM in PP and PEX biofilms. Heating at 55 and 60 °C in copper reactors led to the decline in V. vermiformis and L. pneumophila below the qPCR quantification limit. A rebound of culturable L. pneumophila was noted after a 6 month stagnation period at room temperature in water with PVC and stainless steel coupons.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.006
Threshold uncertainty score0.525

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.235
Teacher spread0.221 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it