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Record W4389257575 · doi:10.1101/2023.12.01.568979

Structure, interaction, and nervous connectivity of beta cell primary cilia

2023· preprint· en· W4389257575 on OpenAlexaff
Andreas Müller, Nikolai Klena, Song Pang, Leticia Elizabeth Galicia Garcia, Oleksandra Topcheva, Davud Sulaymankhil, Monika Seliskar, Hassan Mziaut, Eyke Schöniger, Daniela Friedland, Nicole Kipke, Susanne Kretschmar, Carla Münster, Jürgen Weitz, Marius Distler, Thomas Kurth, Deborah Schmidt, Harald F. Hess, C. Shan Xu, Gaia Pigino, Michele Solimena

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2023
Typepreprint
Languageen
FieldMedicine
TopicPancreatic function and diabetes
Canadian institutionsInnovation Cluster (Canada)
FundersBundesministerium für Bildung und ForschungUppsala UniversitetGerman-Israeli Foundation for Scientific Research and DevelopmentDeutsche ForschungsgemeinschaftEuropean CommissionTechnische Universität DresdenAgence Nationale de la RechercheHoward Hughes Medical InstituteJuvenile Diabetes Research Foundation United States of AmericaLeona M. and Harry B. Helmsley Charitable TrustEuropean Federation of Pharmaceutical Industries and Associations
KeywordsCiliumAxonemeCell biologyIsletParacrine signallingBeta cellIntraflagellar transportBiologyAutocrine signallingBETA (programming language)CellDyneinOrganelleMotile ciliumNeuroscienceMicrotubuleFlagellumCell cultureReceptorEndocrinologyGene

Abstract

fetched live from OpenAlex

Primary cilia are sensory organelles present in many cell types. Based on an array of microtubules termed axoneme they form a specialized membrane compartment partaking in various signaling processes. Primary cilia of pancreatic islet beta cells play a role in autocrine and paracrine signaling and are linked to diabetes. Yet, the structural basis for their functions is unclear. We present three-dimensional reconstructions of complete mouse and human beta cell cilia, revealing a disorganized 9+0 axoneme structure. Within the islet, cilia are spatially confined within deep ciliary pockets or squeezed into narrow extracellular spaces between adjacent cells. Beta and alpha cell cilia physically interact with neighboring islet cells pushing and strongly bending their plasma membranes. Furthermore, beta cells can contain multiple cilia that can meet with other islet cell cilia in the extracellular space. Additionally, beta cell cilia establish connections with islet-projecting nerves. These findings highlight the pivotal role of beta cell primary cilia in islet cell connectivity, pointing at their potential functional role in integrating islet intrinsic and extrinsic signals. These novel insights contribute to understanding their significance in health and diabetes.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.228
Teacher spread0.211 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations10
Published2023
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)→Same topicPancreatic function and diabetes→French-language works237,207→