P0016 PP NUTRIENT‐RELATED PROPHYLAXIS FOR INTESTINAL ISCHEMIA
Bibliographic record
Abstract
Introduction: Intestinal injury as a result of ischemia and subsequent reperfusion plays a key role in various surgical procedures and clinical conditions: aortic aneurysm repair, shock, small bowel transplantation, and neonatal necrotizing enterocolitis.Luminal preservation solution prevents injury during ischemia and facilitates the regeneration of the epithelial barrier upon reperfusion.An amino acid-based luminal solution has been recently developed in our lab that has demonstrated a protective effect during periods of transient intestinal ischemia.This study was designed to examine the role of this novel solution in ameliorating intestinal injury in an in-vivo model of ischemiareperfusion.Methods: SB of Sprague-Dawley rats (N=12) were randomly assigned for luminal pretreatment with the following solutions: Group 1 -none (control); Group 2 -Lactated Ringer's solution; Group 3 -an amino acid (AA) based solution.Warm ischemia (WI) was induced via superior mesenteric artery occlusion for 60 min.Energetics, end products, oxidative stress, histology and barrier function/electrophysiology were assessed after 0, 15, 30 and 60 min reperfusion.Results: ATP and total adenylates were better preserved in Group 3 throughout 60 min reperfusion.Ammonia (deamination product) in Group 3 was 40-85 % higher than Groups1&2after WI.Levels cleared within 15 min reperfusion and remained not significantly different from Groups1&2throughout the remainder of the time-course.Elevations of ammonia and alanine in combination with substantial increases in alanine aminotransferase and glutaminase activity implicated greater amino acid metabolism in AA-treated tissues.Only Group 3 tissues exhibited elevated malondialdehyde (MDA) after WI; values were 40% greater than Groups1&2.After 15 min reperfusion, MDA levels resumed values that similar to Groups1&2 .Glutathione levels were consistently elevated in treated tissues and by 60 min reperfusion values were 6 fold greater than in control.Neutrophil recruitment (assessed by myeloperoxidase activity) was markedly reduced over 30 min reperfusion in Group 3. Barrier function and electrophysiology parameters following IR exhibited a clear pattern of preserved mucosal integrity in tissues treated with AA solution.Histology indicated superior preservation of mucosal architecture in Group 3 tissues after WI and throughout the entire reperfusion period.Conclusion: Pre-ischemic luminal administration of AA solution significantly improves SB quality following ischemiareperfusion.This study raises the possibility of a prophylactic role for luminal nutrient treatment during surgical practices involving the potential complication of intestinal ischemia.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.018 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".