CsCuAOs associate with CsAMADH1 confer drought tolerance through putrescine-derived γ-aminobutyric acid accumulation in tea plants
Bibliographic record
Abstract
Polyamines are potential source of γ-aminobutyric acid (GABA) in plants under abiotic stress. However, the correlation between polyamine degradation and GABA accumulation in tea plants exposed to drought stress is unclear. In the present study, the contents of GABA and putrescine in tea leaves were increased significantly under drought stress, but the glutamate level did not change. At the same time, five copper-containing amine oxidase ( CuAO ) and eight aminoaldehyde dehydrogenase ( AMADH ) genes involved in putrescine-derived GABA pathway were identified from Tea Plant Information Archive. Expression analysis indicated that CsCuAO1 , CsCuAO3, as well as CsAMADH1 were induced to play important function in response to exogenous putrescine or under drought stress. Thus, the three genes were cloned and their soluble recombinant proteins were produced in Escherichia coli . The catalytic efficiency, the pH and temperature optima of them were determined. CsCuAOs and CsAMADH1 exhibited indispensable function in the GABA production from putrescine in vitro . Subcellular localization assays indicated that CsAMADH1 was localized in plastid, while both CsCuAO1 and CsCuAO3 were localized in peroxisome. In addition, the synergistic effects of CsCuAOs and CsAMADH1 were investigated by transient co-expression system in Nicotiana benthamiana . The overexpression of CsAMADH1 lines in Arabidopsis showed increased GABA level supplemented with exogenous putrescine. Our data suggests that these three genes participate the GABA production through putrescine degradation in tea plants that associate with the response to drought stress.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".