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Record W4391656870 · doi:10.1136/gutjnl-2023-330521

Faecal microbial transfer and complex carbohydrates mediate protection against COPD

2024· article· en· W4391656870 on OpenAlexaff
Kurtis F. Budden, Shakti D. Shukla, Kate L Bowerman, Annalicia Vaughan, Shaan L. Gellatly, David Wood, Nancy Lachner, Sobia Idrees, Saima Firdous Rehman, Alen Faiz, Vyoma K. Patel, Chantal Donovan, Charlotte A. Alemao, Sj Shen, Nadia Amorim, Rajib Majumder, Kanth Swaroop Vanka, Jazz Mason, Tatt Jhong Haw, Bree J. Tillet, Michael Fricker, Simon Keely, Nicole G. Hansbro, Gabrielle T. Belz, Jay C. Horvat, Thomas M. Ashhurst, Caryn van Vreden, Helen M. McGuire, Barbara Fazekas de St Groth, Nicholas J. C. King, Ben Crossett, Stuart J. Cordwell, Lorenzo Bonaguro, Joachim L. Schultze, Emma E. Hamilton‐Williams, Elizabeth R. Mann, Samuel C. Forster, Matthew A Cooper, Leopoldo N. Segal, Sanjay H. Chotirmall, Peter Collins, Rayleen Bowman, Kwun M. Fong, Ian A. Yang, Peter Wark, Paul G. Dennis, Philip Hugenholtz, Philip M. Hansbro

Bibliographic record

VenueGut · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGut microbiota and health
Canadian institutionsIONICS Mass Spectrometry (Canada)
FundersNational Health and Medical Research CouncilMedical Research CouncilBundesministerium für Bildung und ForschungPrince Charles Hospital FoundationUniversity of Technology SydneyDeutsche ForschungsgemeinschaftNewcastle University
KeywordsCOPDMicrobiomeImmunologyMedicinePathogenesisGut floraLungInflammationInflammatory bowel diseasePathologyDiseaseBiologyInternal medicineBioinformatics

Abstract

fetched live from OpenAlex

Objective Chronic obstructive pulmonary disease (COPD) is a major cause of global illness and death, most commonly caused by cigarette smoke. The mechanisms of pathogenesis remain poorly understood, limiting the development of effective therapies. The gastrointestinal microbiome has been implicated in chronic lung diseases via the gut-lung axis, but its role is unclear. Design Using an in vivo mouse model of cigarette smoke (CS)-induced COPD and faecal microbial transfer (FMT), we characterised the faecal microbiota using metagenomics, proteomics and metabolomics. Findings were correlated with airway and systemic inflammation, lung and gut histopathology and lung function. Complex carbohydrates were assessed in mice using a high resistant starch diet, and in 16 patients with COPD using a randomised, double-blind, placebo-controlled pilot study of inulin supplementation. Results FMT alleviated hallmark features of COPD (inflammation, alveolar destruction, impaired lung function), gastrointestinal pathology and systemic immune changes. Protective effects were additive to smoking cessation, and transfer of CS-associated microbiota after antibiotic-induced microbiome depletion was sufficient to increase lung inflammation while suppressing colonic immunity in the absence of CS exposure. Disease features correlated with the relative abundance of Muribaculaceae, Desulfovibrionaceae and Lachnospiraceae family members. Proteomics and metabolomics identified downregulation of glucose and starch metabolism in CS-associated microbiota, and supplementation of mice or human patients with complex carbohydrates improved disease outcomes. Conclusion The gut microbiome contributes to COPD pathogenesis and can be targeted therapeutically.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.530
Threshold uncertainty score0.369

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.248
Teacher spread0.232 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations61
Published2024
Admission routes1
Has abstractyes

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