Regulation of Megalin and Clathrin-Mediated Endocytosis by Amp-Activated Protein Kinase
Bibliographic record
Abstract
AMP-activated protein kinase (AMPK) is a metabolic stress sensor, activated at low ATP levels. Our previous studies found AMPK activation reduced cell surface β1-integrin levels which are regulated by clathrin-mediated endocytosis (CME), an internalization mechanism dependent on adaptor proteins and triggered by cytosolic motif of a protein. This indicates that metabolic stress influences expression of some cell surface proteins, likely as a result of AMPK signaling regulating endocytosis. Integrin and Megalin both contain Dab2 recruiting NPXY motif and may both be regulated through AMPK. I confirmed that AMPK activation increased Dab2 recruitment to clathrin structures at the cell surface but decreased when ARFGAP3 and Arf6 are silenced. Using a transgenic LLC-PK1 cell-line I was able to observe increased levels of miniMegalin on cell surface after AMPK stimulation. In conclusion, this work highlights AMPK regulation of endocytic membrane traffic and that it varies depending on the cell type.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".