Dialysis preserves heart function during ex situ heart perfusion
Bibliographic record
Abstract
BackgroundEx situ heart perfusion (ESHP) has been used to optimize donor organs before heart transplantation. However, cardiac function often deteriorates with the development of myocardial edema. The use of dialysis during ESHP could assist in cardiac preservation.MethodsMale Yorkshire pig hearts were subjected to ESHP for 8 hours with or without dialysis. Hearts were supported during nonworking mode and working mode, and pressure-volume loops and coronary vasomotor function were evaluated. Finally, tissue biopsies were assessed for mitochondrial function, oxidative stress, and inflammation.ResultsAdding dialysis to ESHP significantly enhanced cardiac function, with improved preload recruitable stroke work at 4 hours (64.09 ± 20.13 vs 35.08 ± 13.52, p = 0.010) and 8 hours (64.31 ± 9.08 vs 23.30 ± 19.25, p = 0.0002), maximal elastance at 8 hours (24.67 ± 10.75 vs 10.62 ± 8.471, p = 0.0477), and end diastolic pressure volume relationship at 8 hours (644.7 ± 566.68 vs 86.63 ± 72.05, p = 0.0187). Coronary vasomotor function improved in the dialysis group in endothelium dependent (LogIC50 −7.39 ± 0.25 vs −2.22 ± 0.76, p < 0.0001) and independent (LogIC50 −6.11 ± 0.19 vs −4.79 ± 0.11, p < 0.0001) vasorelaxation. Dialyzed hearts also had reduced sensitivity to endothelin-1 (LogEC50 −7.94 ± 0.5 vs −8.54 ± 0.06, p = 0.0449) and significant changes in endothelin receptor-related protein expression related and oxidative stress.ConclusionsThe combination of dialysis with ESHP improves myocardial and coronary vasomotor preservation and may allow for longer perfusion times. Ex situ heart perfusion (ESHP) has been used to optimize donor organs before heart transplantation. However, cardiac function often deteriorates with the development of myocardial edema. The use of dialysis during ESHP could assist in cardiac preservation. Male Yorkshire pig hearts were subjected to ESHP for 8 hours with or without dialysis. Hearts were supported during nonworking mode and working mode, and pressure-volume loops and coronary vasomotor function were evaluated. Finally, tissue biopsies were assessed for mitochondrial function, oxidative stress, and inflammation. Adding dialysis to ESHP significantly enhanced cardiac function, with improved preload recruitable stroke work at 4 hours (64.09 ± 20.13 vs 35.08 ± 13.52, p = 0.010) and 8 hours (64.31 ± 9.08 vs 23.30 ± 19.25, p = 0.0002), maximal elastance at 8 hours (24.67 ± 10.75 vs 10.62 ± 8.471, p = 0.0477), and end diastolic pressure volume relationship at 8 hours (644.7 ± 566.68 vs 86.63 ± 72.05, p = 0.0187). Coronary vasomotor function improved in the dialysis group in endothelium dependent (LogIC50 −7.39 ± 0.25 vs −2.22 ± 0.76, p < 0.0001) and independent (LogIC50 −6.11 ± 0.19 vs −4.79 ± 0.11, p < 0.0001) vasorelaxation. Dialyzed hearts also had reduced sensitivity to endothelin-1 (LogEC50 −7.94 ± 0.5 vs −8.54 ± 0.06, p = 0.0449) and significant changes in endothelin receptor-related protein expression related and oxidative stress. The combination of dialysis with ESHP improves myocardial and coronary vasomotor preservation and may allow for longer perfusion times.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".