First Report of Strawberry Polerovirus 1 in Strawberry in South Korea

HomePlant DiseaseAhead of PrintFirst Report of Strawberry Polerovirus 1 in Strawberry in South Korea Next DISEASE NOTE OPENOpen Access licenseFirst Report of Strawberry Polerovirus 1 in Strawberry in South KoreaMesele Tilahun Belete, Se Eun Kim, Jeong A. Kwon, Ryung-hee Kim, Young-Hwan Kim, Hee-Ji Yang, Mi-Chi Yea, and Jae Sun MoonMesele Tilahun Beletehttps://orcid.org/0000-0002-1504-1804Biosystem and Bioengineering Program, University of Science and Technology (UST), Daejeon 34141, Republic of KoreaPlant System Engineering Research Center, Korean Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaPlant Biotechnology Research Division, Amhara Agricultural Research Institute, Bahir Dar, Ethiopia, Se Eun Kimhttps://orcid.org/0000-0001-5789-5819Biosystem and Bioengineering Program, University of Science and Technology (UST), Daejeon 34141, Republic of Korea, Jeong A. KwonBiosystem and Bioengineering Program, University of Science and Technology (UST), Daejeon 34141, Republic of KoreaPlant System Engineering Research Center, Korean Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea, Ryung-hee KimDepartment of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea, Young-Hwan Kimhttps://orcid.org/0009-0000-5002-8755Department of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea, Hee-Ji YangDepartment of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea, Mi-Chi YeaDepartment of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea, and Jae Sun Moon†Corresponding author: J. S. Moon; E-mail Address: [email protected]Biosystem and Bioengineering Program, University of Science and Technology (UST), Daejeon 34141, Republic of KoreaPlant System Engineering Research Center, Korean Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaAffiliationsAuthors and Affiliations Mesele Tilahun Belete1 2 3 Se Eun Kim1 Jeong A. Kwon1 2 Ryung-hee Kim4 Young-Hwan Kim4 Hee-Ji Yang4 Mi-Chi Yea4 Jae Sun Moon1 2 † 1Biosystem and Bioengineering Program, University of Science and Technology (UST), Daejeon 34141, Republic of Korea 2Plant System Engineering Research Center, Korean Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea 3Plant Biotechnology Research Division, Amhara Agricultural Research Institute, Bahir Dar, Ethiopia 4Department of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea Published Online:28 Feb 2024https://doi.org/10.1094/PDIS-08-23-1706-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleStrawberry (Fragaria × ananassa Duch.) production in South Korea has experienced a steady increase in recent years, primarily driven by the growing consumer demand for fresh strawberries. However, strawberry farmers have faced challenges in the form of severe virus disease epidemics, which have recently resulted in a significant reduction in yield (Kwon et al. 2019; Torrico et al. 2018). More than 30 viruses have been identified to infect strawberries, and among them, strawberry mild yellow edge virus (SMYEV), strawberry mottle virus, and strawberry crinkle virus are considered the most economically significant worldwide and in Korea because of their transmission by aphids (Kwon et al. 2019). Symptoms caused by these viruses include vein banding, stunted growth, and yellowing and curling of leaves (Cho et al. 2011; Kwon et al. 2019). A study was initiated to detect viral agents in strawberry seedlings imported from France, the United States, and the Netherlands. Total RNA was extracted from 52 pooled samples collected from the three countries using the HiYield Total RNA Mini Kit (RBC Bioscience, Taipei, Taiwan), followed by high-throughput sequencing (HTS) as described (Belete et al. 2023) on strawberries grown in isolation from postentry quarantine samples. A total of 556,428,830 trimmed reads were de novo assembled into 153,823 contig sequences with an average contig length of 613.26, ranging from 201 to 21,828 nucleotides. A BLAST search identified several viruses, including SMYEV, dahlia mosaic virus, cucumber mosaic virus, and rose yellow vein virus. In addition, four contigs, ranging from 255 to 455 nucleotides, were 98 to 100% identical to the complete genomic sequence of the strawberry polerovirus 1 (SPV1) isolate AB5301 (GenBank accession no. NC_025435) from a Fragaria × ananassa host from eastern Canada. Leaves of strawberry cultivar Seolhyang with mottling and curling were collected in Nonsan (n = 6), Daejeon (n = 4), Goesan (n = 3), and Seongju (n = 2) provinces in the fall of 2023 and tested by reverse transcription (RT)-PCR with primers designed from the HTS-derived sequence. The previous primer pair SPV1-F/SPV1-R (Kwak et al. 2022) was employed to selectively amplify a specific region of the P1-P2 fusion protein, leading to the generation of a 263-bp product. SPV1 was found in all of the Nonsan and three of the Daejeon samples. The RT-PCR products were confirmed by Sanger sequencing, which showed 100% identity with the HTS-derived sequence. To ascertain the complete genome of SPV1, a set of seven partially overlapping primer pairs were synthesized from the reference complete genome (accession no. NC_025435). Subsequently, RT-PCR was performed using these primers to generate sequencing data. The amplified products were cloned into the RBC-TA vector using vector-specific primers and subjected to Sanger sequencing. DNAMAN software (version 5.2.10; Lynnon Biosoft, Quebec, Canada) was employed to assemble and integrate all the overlapping fragments of the sequence. After completion and necessary nucleotide corrections, the entire 5,986-nt genomic sequence of SPV1 was deposited in the GenBank (accession no. OR295467). Strawberry polerovirus 1 belongs to the genus Polerovirus and the family Solemoviridae and was originally discovered in eastern Canada (Xiang et al. 2015). Subsequently, it has been reported in the United States, Argentina, and more recently in Nepal (Kwak et al. 2022). To our knowledge, this is the first report of SPV1 infecting strawberries in Korea and is significant for the production of virus-free strawberry seedlings.The author(s) declare no conflict of interest.References:Belete, M. T., et al. 2023. Arch. Virol. 168:141. https://doi.org/10.1007/s00705-023-05734-5 Crossref, Google ScholarCho, J.-D., et al. 2011. Plant Pathol. J. 27:187. https://doi.org/10.5423/ppj.2011.27.2.187 Crossref, ISI, Google ScholarKwak, H.-R., et al. 2022. Plant Dis. 106:3006. https://doi.org/10.1094/pdis-02-22-0413-pdn Link, ISI, Google ScholarKwon, S.-J., et al. 2019. Res. Plant Dis. 25:226. https://doi.org/10.5423/rpd.2019.25.4.226 Crossref, Google ScholarTorrico, A. K., et al. 2018. Eur. J. Plant Pathol. 150:983. https://doi.org/10.1007/s10658-017-1337-z Crossref, ISI, Google ScholarXiang, Y., et al. 2015. Arch. Virol. 160:553. https://doi.org/10.1007/s00705-014-2267-0 Crossref, ISI, Google ScholarFunding: This work was supported by a grant (project no. PQ20224B009) from the Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Just PublishedSubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Published: 28 Feb 2024Accepted: 26 Dec 2023 Information© 2024 The American Phytopathological SocietyFundingAnimal and Plant Quarantine AgencyGrant/Award Number: PQ20224B009KeywordsPolerovirusSouth KoreaSPV-1strawberryThe author(s) declare no conflict of interest.PDF download

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