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Record W4392452901 · doi:10.1021/acsanm.3c06040

Cellulose Nanocrystals Protect Plants from Pathogen Infection

2024· article· en· W4392452901 on OpenAlexfundno aff
Henry Squire, M. Kim, Cerise Wong, Amanda Seng, Autumn Y. Lee, Natalie S. Goh, J. Wang, Markita P. Landry

Bibliographic record

VenueACS Applied Nano Materials · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant tissue culture and regeneration
Canadian institutionsnot available
FundersFFAR Fellowship ProgramU.S. Department of AgricultureAlfred P. Sloan FoundationOffice of ScienceCamille and Henry Dreyfus FoundationBurroughs Wellcome FundPhilomathia FoundationGordon and Betty Moore FoundationU.S. Department of DefenseNational Institutes of HealthNational Science FoundationSchmidt Futures
KeywordsPathogenCelluloseNanocrystalMicrobiologyChemistryNanotechnologyMaterials scienceBiologyBiochemistry

Abstract

fetched live from OpenAlex

Plants are capable of mounting an immune defense in response to infection, wounding, or environmental stress. The immune response of a plant is often mediated through the recognition of conserved molecular motifs associated with stress. Treating plants with these molecular motifs prior to exposure to stress, termed immune priming, can improve plant resilience to subsequent stressors. Here, we demonstrate the use of cellulose nanocrystals (CNCs) as an immune priming tool for Arabidopsis thaliana combining phenotypic and transcriptomic data to validate the efficacy of CNCs as an immune primer. Pretreatment of Arabidopsis with CNCs reduces the level of infection by the pathogen Pseudomonas syringae up to 65%. RNA sequencing shows that treatment of Arabidopsis with CNCs results in deep transcriptional reprogramming, perturbing over 1300 genes, most of which are associated with immune regulation. We hypothesize plants recognize CNCs as cell-wall damage, potentially explaining the observed immune response. Finally, physiological characterization of the plant response to CNCs demonstrates minimal effects on plant growth without inducing callose deposition or generation of reactive oxygen species. This work provides a foundation for future investigation of cellulose-based nanomaterials as immune primers, particularly in crop species. Given their ease of synthesis, low cost, degradability, and bio/environmental compatibility, cellulose-based materials could serve as a complement to conventional biocides for pathogen control.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.152
Threshold uncertainty score0.540

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.219
Teacher spread0.210 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations4
Published2024
Admission routes1
Has abstractyes

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