Alfaxalone does not have long‐term effects on goldfish pyramidal neuron action potential properties or <scp> GABA <sub>A</sub> </scp> receptor currents
Bibliographic record
Abstract
Anesthetics have varying physiological effects, but most notably alter ion channel kinetics. Alfaxalone is a rapid induction and washout neuroactive anesthetic, which potentiates γ‐aminobutyric acid (GABA)‐activated GABA A receptor (GABA A ‐R) currents. This study aims to identify any long‐term effects of alfaxalone sedation on pyramidal neuron action potential and GABA A ‐R properties, to determine if its impact on neuronal function can be reversed in a sufficiently short timeframe to allow for same‐day electrophysiological studies in goldfish brain. The goldfish ( Carassius auratus ) is an anoxia‐tolerant vertebrate and is a useful model to study anoxia tolerance mechanisms. The results show that alfaxalone sedation did not significantly impact action potential properties. Additionally, the acute application of alfaxalone onto naive brain slices caused the potentiation of whole‐cell GABA A ‐R current decay time and area under the curve. Following whole‐animal sedation with alfaxalone, a 3‐h wash of brain slices in alfaxalone‐free saline, with saline exchanged every 30 min, was required to remove any potentiating impact of alfaxalone on GABA A ‐R whole‐cell currents. These results demonstrate that alfaxalone is an effective anesthetic for same‐day electrophysiological experiments with goldfish brain slices.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".