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Record W4392927951 · doi:10.32920/25417279.v1

Signalling System Heterogeneity in E. Coli and Shigella

2024· preprint· en· W4392927951 on OpenAlexaff
Raymond Huynh

Bibliographic record

Venuenot available
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicAntibiotic Resistance in Bacteria
Canadian institutionsToronto Metropolitan University
Fundersnot available
KeywordsBiologyEscherichia coliGeneShigella flexneriOpen reading frameGeneticsEngineeringMicrobiologyPeptide sequence

Abstract

fetched live from OpenAlex

The connector protein, PmrD links the PhoPQ and PmrAB two component systems and is critical for PhoPQ-regulated host defense peptide resistance in Escherichia coli. In E. coli the pmrD gene contains three putative start codons near a Shine-Dalgarno sequence and E. coli full-length in-frame PmrD is produced from the second putative start site. In S. flexneri, the pmrD gene contains a 1 bp insertion at the 5' end of the gene, that shifts the open reading frame to the first putative start site. Here, I demonstrate that the start site spacing found in Shigella flexneri does not produce PmrD and that this organism does not undergo PhoPQ regulated polymyxin B resistance. I further show that PmrD in S. sonnei is like that from E. coli and is required for high level PhoPQ-regulated polymyxin B resistance in this organism, suggesting that these species differ in this signaling network.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.002

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.263
Teacher spread0.249 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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