Nickel and FLAG Pull-Down Results From HTT With Putative Interaction Partners
Bibliographic record
Abstract
Huntington’s disease (HD) is a progressive neurological disorder caused by a mutation in the huntingtin gene, which encodes the huntingtin protein. Last year, the first structure of this protein was published. With a global resolution of approximately 4 Angstroms, this was a fantastic leap forward in our understanding of this protein. This structure helped our knowledge of Huntingtin, but there is still much more to find out. The structure published by this group is the only representative of about 75% of the protein, the other 25% being too mobile to capture using many high-resolution mapping techniques. One such mobile area is Exon 1, the location of the triplet repeat expansion responsible for HD. This critical region of the protein requires more structural information to understand. To obtain higher resolution images of this region, we are looking for huntingtin interaction partners that bind this region. These interaction partners will hopefully stabilize the structure enough to image the area. Interaction partners will be verified by a Pull-Down assay. Utilizing the FLAG-Tag and His-Tag present on HuntingtinPolyQ54+HAP40(HTT) complex used in this assay, we will look to identify reliable interaction partners.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.006 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; both teacher heads agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".