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Record W4395037257 · doi:10.1083/jcb.202310006

UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope

2024· article· en· W4395037257 on OpenAlexfundno aff
Yongrong Liao, Leonid Andronov, Xiao-Tian Liu, Junyan Lin, Lucile Guerber, Linjie Lu, Arantxa Agote‐Arán, Evanthia Pangou, Li Ran, Charlotte Kleiss, Mengdi Qu, Stéphane Schmucker, Luca Cirillo, Zhirong Zhang, Daniel Riveline, Monica Gotta, Bruno P. Klaholz, Izabela Sumara

Bibliographic record

VenueThe Journal of Cell Biology · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicNuclear Structure and Function
Canadian institutionsnot available
FundersInstituto Nacional do Câncer, Ministério da SaúdeInstitut National Du CancerInstitute of GeneticsSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen ForschungFrench Infrastructure for Integrated Structural BiologyChina Scholarship CouncilFondation pour la Recherche MédicaleAustralian Research CouncilUniversité de GenèveAgence Nationale de la RechercheFondation ARC pour la Recherche sur le CancerSociété d'Accélération du Transfert de TechnologiesCentre National de la Recherche ScientifiqueSanofi
KeywordsNuclear poreNucleoporinCell biologyBiogenesisCytoplasmNuclear transportNuclear proteinFunction (biology)Scaffold proteinChemistryCell nucleusBiophysicsBiologyTranscription factorBiochemistryGeneSignal transduction

Abstract

fetched live from OpenAlex

Assembly of macromolecular complexes at correct cellular sites is crucial for cell function. Nuclear pore complexes (NPCs) are large cylindrical assemblies with eightfold rotational symmetry, built through hierarchical binding of nucleoporins (Nups) forming distinct subcomplexes. Here, we uncover a role of ubiquitin-associated protein 2-like (UBAP2L) in the assembly and stability of properly organized and functional NPCs at the intact nuclear envelope (NE) in human cells. UBAP2L localizes to the nuclear pores and facilitates the formation of the Y-complex, an essential scaffold component of the NPC, and its localization to the NE. UBAP2L promotes the interaction of the Y-complex with POM121 and Nup153, the critical upstream factors in a well-defined sequential order of Nups assembly onto NE during interphase. Timely localization of the cytoplasmic Nup transport factor fragile X-related protein 1 (FXR1) to the NE and its interaction with the Y-complex are likewise dependent on UBAP2L. Thus, this NPC biogenesis mechanism integrates the cytoplasmic and the nuclear NPC assembly signals and ensures efficient nuclear transport, adaptation to nutrient stress, and cellular proliferative capacity, highlighting the importance of NPC homeostasis at the intact NE.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.771
Threshold uncertainty score0.369

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.229
Teacher spread0.221 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations6
Published2024
Admission routes1
Has abstractyes

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