517-P: Postprandial Circulating Endocannabinoidome Mediators—Impact of Host's Metabolic Health and Dietary Fatty Acid Profile
Bibliographic record
Abstract
Introduction & Objective: Food digestion and the activity of the gut microbiota enable the production of several nutrient-derived bioactive metabolites. Notably, lipids can be metabolized into endocannabinoidome (eCBome) mediators, a large family of endocannabinoid and their congeners such as N-acyl-ethanolamines (NAEs) and 2-monoacyl-glycerols (2-MAGs) involved in numerous aspects of energy homeostasis. Our objective is to decipher the short-term and postprandial kinetics of dietary fatty acid incorporation into circulating eCBome mediators in individuals living with visceral obesity and type 2 diabetes (T2D). Methods: The METAc trial consists of a fixed sequence of standardized meals divided in three periods: a) a 3-day run-in control diet, b) a 2-day UFA-rich diet, and c) a 2-day SFA-rich diet. Fasting and postprandial (45-480 min) circulating levels of NAEs and 2-MAGs were measured by LC-MS/MS and analyzed using longitudinal mixed linear models. Results: Men and postmenopausal women aged 45 to 65 (n=51) were recruited into the following groups: lean, visceral obesity and T2D. Circulating levels of 2-MAGs derived from oleic acid and omega‑3 PUFAs were increased following the 2-day UFA-rich diet (+25 to 120%). Interestingly, circulating levels of all 2-MAGs were rapidly increased along with digestion (+40 to 220%). In contrast, circulating levels of NAEs were decreased throughout the postprandial periods (−40 to 80%). The magnitude of the postprandial response of most NAE and 2-MAG congeners was influenced by the fatty acid profile of the previous meal. In addition, the eCBome profile was impacted by the obesity and T2D status of the host. Conclusion: This study supports the hypothesis that dietary lipids are rapidly metabolized and incorporated into circulating 2-MAGs. Dietary lipid intake, obesity and diabetes status as well as gut microbiota activity emerge as crucial regulators of the fasting and postprandial circulating eCBome tone. Disclosure T. Rocheleau: None. L. Amzil: None. T. Mayer: None. N. Leblanc: None. N. Flamand: None. F. Raymond: None. V. Di Marzo: None. A. Veilleux: None.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".