Environmental Influences on Frozen Epididymal Sperm Cell Viability in White-Tailed Deer
Bibliographic record
Abstract
The objective of this study was to evaluate the association between Temperature and Humidity Index (THI), age, and post-collection time with post-thawing sperm cell parameters such as progressive motility, viability, morphology, and cytoplasmic droplets. Samples were collected from 4 male white-tailed deer after regulated hunting. THI was calculated on collection days. Two THI categories were determined, ≥70 and <70. Thawed semen was placed on a slide and observed under a phase-contrast microscope to evaluate the progressively motile sperm cells. Morphology, viability, and cytoplasmic droplets were assessed on a smear stained with eosin-nigrosine and classified as normal or abnormal, viable or not viable, and present or absent, respectively. When THI was ≥70, the progressive motility (51.0 vs 30.67%), sperm viability (51.0 vs 36.33%), and the proportion of sperm cells with normal morphology (65.0 vs 37.67%) were higher than to days with a THI <70, without differences in the presence of cytoplasmic droplets. The 4-years bucks had a higher proportion of sperm cells with normal morphology (81.0 vs 35.5 and 26.0%) and cytoplasmic droplets (27.0 vs. 12.0 and 7.5%) compared to 2- and 3-year-old animals. Samples processed after 4 hours (51.0%) had a higher progressive motility than samples processed after 6 and 8 hours (32.0 and 25.0%, respectively). Spermatozoa processed after 8 hours (30.0%) had a lower viability than spermatozoa processed after 4 hours (51.0%). Compared to samples processed after 6 and 8 hours, those processed after 2 and 4 hours had a higher proportion of sperm cells with normal morphology (26.0 and 6.0 vs 81.0 and 65.0%, respectively). Samples processed after 2 hours had a higher (27.0 vs. 12.0%) proportion of sperm cells with cytoplasmic droplets than to those processed after 6 hours. High THI favors progressive motility and sperm viability. The older bucks had a greater proportion of sperm cells with normal morphology and cytoplasmic droplets than to the young ones. The greater progressive motility, viability, morphology, and presence of cytoplasmic droplets were found when the samples were processed within the first 4 hours.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".